PMID- 11502665
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20190514
IS  - 0012-3692 (Print)
IS  - 0012-3692 (Linking)
VI  - 120
IP  - 2
DP  - 2001 Aug
TI  - High concentrations of beta-chemokines in BAL fluid of patients with diffuse 
      panbronchiolitis.
PG  - 602-7
AB  - BACKGROUND: T cells are important cellular components of bronchial inflammation 
      in diffuse panbronchiolitis (DPB). beta-Chemokines such as RANTES (regulated on 
      activation, normal T-cell expressed and secreted) and macrophage inflammatory 
      peptide (MIP)-1alpha are closely related to the migration of inflammatory cells 
      into the lung. In this study, we investigate the contribution of beta-chemokines 
      to the accumulation of T cells in the lungs of patients with DPB. PATIENTS AND 
      METHODS: We determined the levels of beta-chemokines in BAL fluid (BALF) and the 
      correlation between these levels and T-cell subsets in BALF of 23 patients with 
      DPB and 16 healthy subjects by sandwich enzyme-linked immunosorbent assay and 
      flow cytometry. RESULTS: Percentages of CD3+ human leukocyte antigen (HLA)-DR+, 
      CD8+, and CD8+HLA-DR+ cells in BALF of patients were significantly higher than in 
      the control BALF. The absolute number of CD8+HLA-DR+ cells was also higher in 
      BALF of patients than in the control BALF (p < 0.0001). Phenotypic analysis of 
      CD4+ cells in BALF showed a similar percentage of CD4+CD45RA+ cells and CD4+CD29+ 
      cells in patients and normal subjects. The concentrations of RANTES and 
      MIP-1alpha in BALF of patients with DPB were significantly higher than in BALF of 
      normal subjects (p < 0.05). In addition, there was a significant correlation 
      between the absolute number or percentage of CD8+HLA-DR+ cells and MIP-1alpha 
      concentration in BALF. CONCLUSIONS: Our results suggest that the interaction 
      between activated CD8+ T cells and MIP-1alpha may contribute to the pathogenesis 
      of DPB.
FAU - Kadota, J
AU  - Kadota J
AD  - Second Department of Internal Medicine, Nagasaki University School of Medicine, 
      Nagasaki, Japan. kadota@oita-med.ac.jp
FAU - Mukae, H
AU  - Mukae H
FAU - Tomono, K
AU  - Tomono K
FAU - Kohno, S
AU  - Kohno S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bronchiolitis/*metabolism
MH  - Bronchoalveolar Lavage Fluid/*chemistry/cytology
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/analysis
MH  - Chemokines, CC/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Macrophage Inflammatory Proteins/analysis
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocyte Subsets/cytology
EDAT- 2001/08/15 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/15 10:00
PHST- 2001/08/15 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/15 10:00 [entrez]
AID - S0012-3692(15)51474-8 [pii]
AID - 10.1378/chest.120.2.602 [doi]
PST - ppublish
SO  - Chest. 2001 Aug;120(2):602-7. doi: 10.1378/chest.120.2.602.