PMID- 11503207
OWN - NLM
STAT- MEDLINE
DCOM- 20020208
LR  - 20131121
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 1
IP  - 7
DP  - 2001 Jul
TI  - Identification of protein carbonyls after two-dimensional electrophoresis.
PG  - 829-34
AB  - The oxidative modification of proteins plays a major role in a number of human 
      diseases, but identity of the specific proteins that are most susceptible to 
      oxidation has posed a difficult problem. Protein carbonyls are increased after 
      oxidative stress, and after derivatization with 2,4-dinitrophenyl hydrazine (DNP) 
      they can be detected by various analytical and immunological methods. Although 
      high resolution two-dimensional electrophoresis (2-DE) can resolve virtually all 
      proteins present in a cell or tissue it has been difficult to determine the 
      oxidized proteins because the DNP-derivatization process alters the isoelectric 
      points of proteins, and additional procedures must be utilized to remove reaction 
      byproducts. These additional procedures can lead to loss of sample, and poor 
      isoelectric resolution on immobilized pH gradient (IPG) strips. We have developed 
      a method that allows the IPG strips to be derivatized with DNP directly following 
      isoelectric focusing of the proteins. This method allows the visualization of 
      oxidized proteins by 2-DE with high reproducibility.
FAU - Conrad, C C
AU  - Conrad CC
AD  - Molecular Aging Unit, Department of Molecular Biology and Immunology, University 
      of North Texas Health Science Center, Texas, USA.
FAU - Choi, J
AU  - Choi J
FAU - Malakowsky, C A
AU  - Malakowsky CA
FAU - Talent, J M
AU  - Talent JM
FAU - Dai, R
AU  - Dai R
FAU - Marshall, P
AU  - Marshall P
FAU - Gracy, R W
AU  - Gracy RW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Blood Proteins)
RN  - 0 (Phenylhydrazines)
RN  - 1N39KD7QPJ (2,4-dinitrophenylhydrazine)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Blood Proteins/*chemistry/*isolation & purification
MH  - Electrophoresis, Gel, Two-Dimensional/*methods
MH  - Humans
MH  - Isoelectric Focusing/methods
MH  - Molecular Weight
MH  - Oxidative Stress
MH  - Phenylhydrazines
MH  - Silver
MH  - Staining and Labeling
EDAT- 2001/08/16 10:00
MHDA- 2002/02/09 10:01
CRDT- 2001/08/16 10:00
PHST- 2001/08/16 10:00 [pubmed]
PHST- 2002/02/09 10:01 [medline]
PHST- 2001/08/16 10:00 [entrez]
AID - 10.1002/1615-9861(200107)1:7<829::AID-PROT829>3.0.CO;2-R [doi]
PST - ppublish
SO  - Proteomics. 2001 Jul;1(7):829-34. doi: 
      10.1002/1615-9861(200107)1:7<829::AID-PROT829>3.0.CO;2-R.