PMID- 11504553
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 287
IP  - 1
DP  - 2001 Aug 15
TI  - A 10-kDa structural protein of porcine reproductive and respiratory syndrome 
      virus encoded by ORF2b.
PG  - 183-91
AB  - The major structural proteins of porcine reproductive and respiratory syndrome 
      virus (PRRSV) are derived from ORFs 5, 6, and 7. Western blots of sucrose 
      gradient-purified virions and PRRSV-infected MARC-145 cells, probed with immune 
      pig serum, showed the presence of an additional 10-kDa protein. Nucleotide 
      sequence analysis of North American PRRSV isolate SDSU-23983 revealed a small ORF 
      within ORF2, named ORF2b, which, when translated, produced a 73-amino-acid 
      nonglycosylated protein. Recombinant 2b protein expressed by a baculovirus clone, 
      AcVR2, comigrated with the 10-kDa virus-associated protein. The loss of 10-kDa 
      protein immunoreactivity after absorption of immune sera with lysates from 
      AcVR2-infected insect cells demonstrated that the 2b and 10-kDa proteins are 
      immunologically similar. Immunoblots were also used for the detection of anti-2b 
      activity in serum samples from experimentally infected adult pigs. Antibodies 
      against PRRSV were apparent by 14 days postinfection, followed by anti-2b 
      activity and serum neutralizing activity. The putative ORF2b start codon is only 
      6 nucleotides downstream of the adenine of the ORF2a start codon. The expression 
      of ORF2a and 2b as enhanced green fluorescent fusion proteins showed that both 
      proteins were translated; however, the ORF2b was preferentially expressed. These 
      results suggest that the 2b protein is virion associated and the principal 
      product of ORF2.
CI  - Copyright 2001 Academic Press.
FAU - Wu, W H
AU  - Wu WH
AD  - Department of Veterinary Science, South Dakota State University, Brookings, South 
      Dakota 57007, USA.
FAU - Fang, Y
AU  - Fang Y
FAU - Farwell, R
AU  - Farwell R
FAU - Steffen-Bien, M
AU  - Steffen-Bien M
FAU - Rowland, R R
AU  - Rowland RR
FAU - Christopher-Hennings, J
AU  - Christopher-Hennings J
FAU - Nelson, E A
AU  - Nelson EA
LA  - eng
SI  - GENBANK/AF035409
SI  - GENBANK/AF121131
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Baculoviridae
MH  - Base Sequence
MH  - Blotting, Western
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Male
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - *Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Recombinant Proteins/chemistry
MH  - Spodoptera
MH  - Transfection
MH  - Viral Structural Proteins/*genetics
EDAT- 2001/08/16 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/08/16 10:00
PHST- 2001/08/16 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/08/16 10:00 [entrez]
AID - S0042-6822(01)91034-7 [pii]
AID - 10.1006/viro.2001.1034 [doi]
PST - ppublish
SO  - Virology. 2001 Aug 15;287(1):183-91. doi: 10.1006/viro.2001.1034.