PMID- 11504684
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20211203
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 281
IP  - 3
DP  - 2001 Sep
TI  - Differential expression of Tie-2 receptors and angiopoietins in response to in 
      vivo hypoxia in rats.
PG  - L582-90
AB  - In this study, we assessed the effects of in vivo hypoxia on the expression of 
      Tie-2 receptors and angiopoietins in various organs of conscious rats and 
      correlated these effects with the expression of hypoxia-inducible factor-1 
      (HIF-1). RT-PCR and Southern blotting were used to amplify mRNA expression of 
      angiopoietin-1, -2, and -3, Tie-2, and HIF-1 alpha in tissues of normoxic and 
      hypoxic (fraction of inspired oxygen of 9--10% for either 12 or 48 h) rats. 
      Hypoxia provoked a decline in angiopoietin-1 mRNA and Tie-2 mRNA, protein, and 
      phosphorylation levels in the lung, liver, cerebellum, and heart but not in the 
      kidney and diaphragm. In comparison, hypoxia raised the levels of angiopoietin-2 
      mRNA in the cerebellum and angiopoietin-3 mRNA in the lung, kidney, and 
      diaphragm. HIF-1 alpha mRNA was abundant in most organs of normoxic rats but was 
      significantly induced in the kidney and diaphragm of hypoxic rats. We conclude 
      that in vivo hypoxia exerts inhibitory effects on the activity of the 
      angiopoietin-1/Tie-2 receptor pathway through reduction of angiopoietin-1 and 
      upregulation of angiopoietin-2 and -3. Induction of angiopoietin-3 in the kidney 
      and diaphragm of hypoxic rats could be mediated through the HIF-1 transcription 
      factor.
FAU - Abdulmalek, K
AU  - Abdulmalek K
AD  - Critical Care and Respiratory Divisions, Royal Victoria Hospital, Montreal, 
      Quebec H3A 1A1, Canada.
FAU - Ashur, F
AU  - Ashur F
FAU - Ezer, N
AU  - Ezer N
FAU - Ye, F
AU  - Ye F
FAU - Magder, S
AU  - Magder S
FAU - Hussain, S N
AU  - Hussain SN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (ANGPTL1 protein, human)
RN  - 0 (Angiopoietin-1)
RN  - 0 (Angiopoietin-2)
RN  - 0 (Angiopoietin-Like Protein 1)
RN  - 0 (Angiopoietin-like Proteins)
RN  - 0 (Angiopoietins)
RN  - 0 (Angpt1 protein, rat)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
SB  - IM
MH  - Angiopoietin-1
MH  - Angiopoietin-2
MH  - Angiopoietin-Like Protein 1
MH  - Angiopoietin-like Proteins
MH  - Angiopoietins
MH  - Animals
MH  - Base Sequence/genetics
MH  - Blotting, Southern
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Hypoxia/*metabolism
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Nuclear Proteins/genetics/metabolism
MH  - Proteins/genetics/*metabolism
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, TIE-2
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Transcription Factors
EDAT- 2001/08/16 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/08/16 10:00
PHST- 2001/08/16 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/08/16 10:00 [entrez]
AID - 10.1152/ajplung.2001.281.3.L582 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2001 Sep;281(3):L582-90. doi: 
      10.1152/ajplung.2001.281.3.L582.