PMID- 11505054
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20220311
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 25
IP  - 8
DP  - 2001 Aug
TI  - Early alteration in leukocyte populations and Th1/Th2 function in 
      ethanol-consuming mice.
PG  - 1221-30
AB  - BACKGROUND: Chronic alcohol consumption polarizes the immune response away from 
      Th1-mediated cell-mediated immunity. In the present report we investigate the 
      first onset of alteration in immune parameters during ethanol consumption in 
      terms of changes in splenic leukocyte cellularity and surface phenotype as well 
      as alterations in Th1 and Th2 function. METHODS: BALB/c and C57BL/6 mice were fed 
      ethanol-containing liquid diets, were pair-fed an isocaloric liquid control diet, 
      or were fed solid diet and water ad libitum for up to 12 days. At intervals 
      during the feeding period, splenic leukocytes were assessed for phenotypic 
      markers by flow cytometry and for their ability to support antigen-induced 
      interferon-gamma (IFNgamma) production in a coculture system. Mice were bled at 
      intervals throughout the feeding period, and serum immunoglobin E (IgE) and 
      alcohol levels were determined. RESULTS: Data show that phenotypic and functional 
      alterations occur within the first few days of alcohol consumption. Both liquid 
      diets affect splenic cellularity, and by dietary day 5, ethanol-containing liquid 
      diets further reduce B and NK cell numbers. The decline in B cells is accompanied 
      by a concomitant decline in the amount of major histocompatibility complex class 
      II expressed on this population. Functional alteration in Th1-mediated IFNgamma 
      production occurred in the population fed ethanol-containing liquid diets by 
      dietary day 5. Th2 function, as indicated by systemic serum IgE levels in these 
      unimmunized mice, is increased by dietary day 6 to 8 and correlated with 
      significant blood alcohol levels. CONCLUSIONS: Ethanol consumption by mice causes 
      a rapid decrease in splenic cellularity accompanied by a decrease in Th1 function 
      and a rapid increase in systemic IgE levels.
FAU - Starkenburg, S
AU  - Starkenburg S
AD  - Department of Microbiology-Immunology, Northwestern University School of 
      Medicine, Chicago, Illinois 60611-3073, USA.
FAU - Munroe, M E
AU  - Munroe ME
FAU - Waltenbaugh, C
AU  - Waltenbaugh C
LA  - eng
GR  - AA05527/AA/NIAAA NIH HHS/United States
GR  - AA08275/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3K9958V90M (Ethanol)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Alcoholism/immunology
MH  - Animals
MH  - B-Lymphocytes
MH  - Coculture Techniques
MH  - Ethanol/*administration & dosage
MH  - Female
MH  - Histocompatibility Antigens Class II/analysis
MH  - Immunoglobulin E/blood
MH  - Interferon-gamma/biosynthesis
MH  - Killer Cells, Natural
MH  - *Leukocyte Count
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Self Administration
MH  - Spleen/cytology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
EDAT- 2001/08/16 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/08/16 10:00
PHST- 2001/08/16 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/08/16 10:00 [entrez]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2001 Aug;25(8):1221-30.