PMID- 11506481
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20190915
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 109
IP  - 6
DP  - 2001 Jun
TI  - Investigation of an outbreak caused by methicillin-resistant Staphylococcus 
      aureus in a cardiovascular surgery unit by ribotyping, randomly amplifed 
      polymorphic DNA and pulsed-field gel electrophoresis.
PG  - 474-80
AB  - An outbreak caused by rapid spread of methicillin-resistant Staphylococcus aureus 
      (MRSA) in an intensive care unit for cardiovascular surgery was investigated by 
      phenotypic and genotypic methods. Fourteen isolates were collected during a 
      2-month period from clinical and environmental specimens in the unit recently 
      re-opened after reconstruction. The isolates were tested for antibiotic 
      susceptibility patterns and genotyped by automated ribotyping, randomly amplified 
      polymorphic DNA-PCR (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). 
      Automated ribotyping applying EcoRI digestion proved to be of no value in 
      separating the isolates. In contrast, PFGE grouped the isolates into four 
      clusters different from the reference strain. These results fully correlated with 
      the antibiograms. Twelve of the isolates were grouped into two clonally related 
      clusters. RAPD analyses grouped the isolates into five clusters. Except for two 
      isolates of one patient, which had different RAPD patterns, PFGE and RAPD 
      analyses presented very similar results. The results verified the usefulness of 
      PFGE in studies of MRSA epidemics. A combination of these two methods reduces the 
      time to identification of an outbreak and increases the accuracy in detection of 
      intraspecies differences.
FAU - Fung, C P
AU  - Fung CP
AD  - Department of Medicine, Veterans General Hospital-Taipei, and National Yang-Ming 
      University, Taiwan.
FAU - Ho, M W
AU  - Ho MW
FAU - Wang, F D
AU  - Wang FD
FAU - Tsai, K
AU  - Tsai K
FAU - Liu, C E
AU  - Liu CE
FAU - Liu, C Y
AU  - Liu CY
FAU - Siu, L K
AU  - Siu LK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Base Sequence
MH  - Cardiovascular Surgical Procedures/adverse effects
MH  - Cross Infection/*epidemiology/microbiology
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - *Disease Outbreaks
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Humans
MH  - Intensive Care Units
MH  - *Methicillin Resistance
MH  - Random Amplified Polymorphic DNA Technique
MH  - Ribotyping
MH  - Staphylococcal Infections/*epidemiology/microbiology
MH  - Staphylococcus aureus/classification/*drug effects/*genetics/isolation & 
      purification
MH  - Surgery Department, Hospital
MH  - Taiwan/epidemiology
EDAT- 2001/08/17 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/08/17 10:00
PHST- 2001/08/17 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/08/17 10:00 [entrez]
AID - 10.1034/j.1600-0463.2001.090611.x [doi]
PST - ppublish
SO  - APMIS. 2001 Jun;109(6):474-80. doi: 10.1034/j.1600-0463.2001.090611.x.