PMID- 11506743
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20181130
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 7
DP  - 2001 Jul
TI  - Human monocyte-derived dendritic cells produce bioactive gelatinase B: inhibition 
      by IFN-beta.
PG  - 495-501
AB  - We studied the secretion of gelatinase B by dendritic cells (DC) generated by 
      culturing human peripheral blood monocytes in granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). First, we found the 
      intracellular expression of gelatinase B on sections of fixed DC pellets. 
      Zymography analysis of the supernatants of DC cultured for 72 h demonstrated the 
      presence of gelatinase B. To determine if DC produce net enzymatic activity, 
      bioactive gelatinase, a novel sensitive fluorescent-activated substrate 
      conversion (FASC) assay was used to complement the zymography data. Culture media 
      of unstimulated DC demonstrated reproducible net gelatinolytic activity. Tumor 
      necrosis factor-alpha (TNF-alpha) IL-1beta but not lipopolysaccharide (LPS) 
      stimulation caused a significant increase in gelatinase B production in 
      zymography analysis. Both types of stimulation failed to increase net gelatinase 
      activity in FASC assay. Interestingly, interferon-beta (IFN-beta) significantly 
      diminished both the total zymolytic production and the net bioactive gelatinase 
      produced by DC in a dose-dependent manner. We conclude that human 
      monocyte-derived DC secrete bioactive gelatinase B and that IFN-beta inhibits 
      this production.
FAU - Bartholome, E J
AU  - Bartholome EJ
AD  - Department of Immunology, Hopital Erasme, Universite Libre de Bruxelles, 
      Brussels, Belgium. ebarthol@ulb.ac.be
FAU - Van Aelst, I
AU  - Van Aelst I
FAU - Koyen, E
AU  - Koyen E
FAU - Kiss, R
AU  - Kiss R
FAU - Willems, F
AU  - Willems F
FAU - Goldman, M
AU  - Goldman M
FAU - Opdenakker, G
AU  - Opdenakker G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 77238-31-4 (Interferon-beta)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/*enzymology/immunology/metabolism
MH  - Dose-Response Relationship, Immunologic
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interferon-beta/*pharmacology
MH  - Matrix Metalloproteinase 9/*biosynthesis/metabolism
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Monocytes/*enzymology/immunology
EDAT- 2001/08/17 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/08/17 10:00
PHST- 2001/08/17 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/08/17 10:00 [entrez]
AID - 10.1089/10799900152434367 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Jul;21(7):495-501. doi: 
      10.1089/10799900152434367.