PMID- 11508385
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20211216
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 65
IP  - 2
DP  - 2001 Aug
TI  - Toxins and colonization factor antigens of enterotoxigenic Escherichia coli among 
      residents of Jakarta, Indonesia.
PG  - 120-4
AB  - Infection caused by enterotoxigenic Escherichia coli (ETEC) poses a serious 
      health problem among children and adults in developing countries. Colonization of 
      the small intestinal mucosa by ETEC strains is mediated by antigenically specific 
      fimbriae, also known as colonization factor antigens (CFA). The significance of 
      this study arises from reports that active and passive immunization with ETEC 
      strains harboring CFAs has previously been shown to induce protective immunity 
      against diarrhea in animal models. The aim of this study was to determine 
      toxin-associated CFAs of ETEC isolated from a diarrheal disease case-control 
      study in Jakarta, Indonesia. Thirteen hundred and twenty-three diarrheic and 
      control patients with lactose-fermenting colonies were screened by ganglioside 
      GM1-enzyme-linked immunosorbent assay (GM1-ELISA) for heat-labile (LT) and 
      heat-stable (ST) toxins. Two hundred and forty-six (19%) ETEC isolates identified 
      by GM1-ELISA for the LT/ST toxins were screened for CFAs by Dot blot assay using 
      monoclonal antibodies against CFA/I, II, and IV and against the putative 
      colonization antigens (PCF) PCFO159, PCFO166, CS7, and CS17. Of the 246 ETEC 
      isolates, 177 (72%) elaborated ST, 56 (23%) produced LT, while 13 (5%) elicited 
      both the ST and LT toxins. CFA testing of the 246 ETEC isolates showed that 21 
      (8%) expressed CFA/I, 3 (1%) exhibited CFA/II, 14 (6%) elaborated CFA/IV, while 7 
      (3%) expressed PCFO159 and PCFO159 plus CS5. No CFAs or PCFs could be associated 
      with 201 (82%) of the ETEC strains. This report documents the types of CFAs 
      associated with ETEC strains in Jakarta, Indonesia. These data may help current 
      research efforts on the development of CFA-based vaccines for humans against ETEC 
      and provide additional information for future ETEC vaccine trials in Southeast 
      Asia.
FAU - Oyofo, B A
AU  - Oyofo BA
AD  - United States Naval Medical Research Unit No. 2, Jakarta, Indonesia.
FAU - Subekti, D S
AU  - Subekti DS
FAU - Svennerholm, A M
AU  - Svennerholm AM
FAU - Machpud, N N
AU  - Machpud NN
FAU - Tjaniadi, P
AU  - Tjaniadi P
FAU - Komalarini, T S
AU  - Komalarini TS
FAU - Setiawan, B
AU  - Setiawan B
FAU - Campbell, J R
AU  - Campbell JR
FAU - Corwin, A L
AU  - Corwin AL
FAU - Lesmana, M
AU  - Lesmana M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Gangliosides)
RN  - 0 (colonization factor antigens)
RN  - 0 (heat stable toxin (E coli))
RN  - 147680-16-8 (Fimbriae Proteins)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bacterial Proteins/*analysis
MH  - Bacterial Toxins/*analysis
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Diarrhea/epidemiology/*microbiology
MH  - Enterotoxins/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Escherichia coli/*immunology/isolation & purification/pathogenicity
MH  - Escherichia coli Infections/epidemiology/*microbiology
MH  - *Escherichia coli Proteins
MH  - Feces/microbiology
MH  - Female
MH  - *Fimbriae Proteins
MH  - Gangliosides
MH  - Humans
MH  - Immunoblotting
MH  - Indonesia/epidemiology
MH  - Infant
MH  - Male
MH  - Middle Aged
EDAT- 2001/08/18 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/18 10:00
PHST- 2001/08/18 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/18 10:00 [entrez]
AID - 10.4269/ajtmh.2001.65.120 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2001 Aug;65(2):120-4. doi: 10.4269/ajtmh.2001.65.120.