PMID- 11508576
OWN - NLM
STAT- MEDLINE
DCOM- 20020125
LR  - 20220311
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 28
IP  - 8
DP  - 2001 Aug
TI  - Tumor necrosis factor-alpha (TNF-alpha) converting enzyme contributes to 
      production of TNF-alpha in synovial tissues from patients with rheumatoid 
      arthritis.
PG  - 1756-63
AB  - OBJECTIVE: Expression and function of tumor necrosis factor-alpha (TNF-alpha) 
      converting enzyme (TACE) in synovia of patients with rheumatoid arthritis (RA) 
      were examined to investigate posttranslational regulation of TNF-alpha production 
      by TACE in RA. METHODS: Expression of TACE protein was evaluated by 
      immunohistochemistry. Cytokines and soluble cytokine receptors were measured by 
      ELISA. TACE mRNA was detected by RT-PCR. The enzymatic activity of TACE was 
      measured using TACE-specific fluorogenic substrate. RESULTS: Expression of TACE 
      at protein level in synovial tissue (ST) of patients with RA was significantly 
      stronger than that of patients with osteoarthritis (OA). In RA, TACE was mainly 
      expressed in CD68+ macrophage-like synovial cells. ST from 9 of 9 RA and 3 of 8 
      OA patients expressed TACE mRNA. RA ST cells possessed significantly higher 
      TACE-like enzymatic activity than OA ST. A synthetic TACE inhibitor significantly 
      reduced the release of TNF-alpha and p75 TNF receptor from RA ST cells. 
      CONCLUSION: TACE is an important regulator of the secretion of TNF-alpha from 
      synovia of patients with RA.
FAU - Ohta, S
AU  - Ohta S
AD  - Institute of Rheumatology, Tokyo Women's Medical University, Japan.
FAU - Harigai, M
AU  - Harigai M
FAU - Tanaka, M
AU  - Tanaka M
FAU - Kawaguchi, Y
AU  - Kawaguchi Y
FAU - Sugiura, T
AU  - Sugiura T
FAU - Takagi, K
AU  - Takagi K
FAU - Fukasawa, C
AU  - Fukasawa C
FAU - Hara, M
AU  - Hara M
FAU - Kamatani, N
AU  - Kamatani N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
SB  - IM
MH  - ADAM Proteins
MH  - ADAM17 Protein
MH  - Arthritis, Rheumatoid/*metabolism/pathology/physiopathology
MH  - Frozen Sections
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Immunohistochemistry
MH  - Metalloendopeptidases/analysis/*genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Synovial Membrane/*enzymology/immunology/pathology
MH  - Synovitis/metabolism/pathology/physiopathology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 2001/08/18 10:00
MHDA- 2002/01/26 10:01
CRDT- 2001/08/18 10:00
PHST- 2001/08/18 10:00 [pubmed]
PHST- 2002/01/26 10:01 [medline]
PHST- 2001/08/18 10:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 2001 Aug;28(8):1756-63.