PMID- 1150882
OWN - NLM
STAT- MEDLINE
DCOM- 19751106
LR  - 20220318
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 56
IP  - 2
DP  - 1975 Aug
TI  - Free DNA in serum and plasma from normal adults.
PG  - 512-5
AB  - Circulating DNA has been associated with several human disorders, including the 
      nephritis of systemic lupus erythematosus (SLE), in which it is thought to play 
      an etiological role. However, it remains unclear whether its appearance in the 
      circulation is truly pathological. Several reports, each generally based on a 
      single assay method, have disagreed as to whether DNA may circulate in normals. 
      Some, but not all, of this disagreement may be explained by the recently 
      described appearance of DNA in serum, but not plasma, apparently as the result of 
      release from leukocytes in vitro. In the present report an attempt is made to 
      clarify this problem. Normal plasma and serum samples were examined by four 
      assays for DNA that were newly modified to enhance their specificity and/or 
      sensitivity. Plasma DNA was undetectable by all four methods, the most sensitive 
      of which could detect 0.05 mug/ml of native DNA (nDNA) or 0.1 mug/ml of 
      single-stranded DNA (ssDNA). Serum DNA was present in 14 of 16 samples tested in 
      variable concentrations with an estimated mean of 1.9 mug/ml. It is concluded 
      that the appearance of DNA in adult human plasma is a pathological event. 
      Presumably, previous reports describing detection of DNA in normal plasma were 
      based on the measurement of non-DNAase-sensitive interfering substance. 
      Furthermore, it is emphasized that the use of serum in studies dependent on 
      sensitive assays for DNA (or anti-DNA antibody) introduces an ambiguity that may 
      be avoided by substitution of carefully collected plasma for serum.
FAU - Steinman, C R
AU  - Steinman CR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 9007-49-2 (DNA)
RN  - 9N3CBB0BIQ (Diphenylamine)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Adult
MH  - DNA/*blood
MH  - Diphenylamine
MH  - Ethidium
MH  - Humans
MH  - Methods
PMC - PMC436612
EDAT- 1975/08/01 00:00
MHDA- 1975/08/01 00:01
PMCR- 1975/08/01
CRDT- 1975/08/01 00:00
PHST- 1975/08/01 00:00 [pubmed]
PHST- 1975/08/01 00:01 [medline]
PHST- 1975/08/01 00:00 [entrez]
PHST- 1975/08/01 00:00 [pmc-release]
AID - 10.1172/JCI108118 [doi]
PST - ppublish
SO  - J Clin Invest. 1975 Aug;56(2):512-5. doi: 10.1172/JCI108118.