PMID- 11511752
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20190513
IS  - 1462-0324 (Print)
IS  - 1462-0324 (Linking)
VI  - 40
IP  - 8
DP  - 2001 Aug
TI  - Monoclonal anti-double stranded DNA antibody is a leucocyte-binding protein to 
      up-regulate interleukin-8 gene expression and elicit apoptosis of normal human 
      polymorphonuclear neutrophils.
PG  - 851-8
AB  - OBJECTIVES: To determine whether anti-double stranded DNA (anti-dsDNA) 
      autoantibody could bind and affect the functions of normal human 
      polymorphonuclear neutrophils (PMN). METHODS: Normal human PMN were incubated 
      with different concentrations of a monoclonal mouse anti-dsDNA antibody (12B3) or 
      mouse isotype-matched IgG2a. The binding of anti-dsDNA and PMN was measured by 
      flow cytometry and interleukin-8 (IL-8) gene expression in PMN was detected by 
      enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase 
      chain reaction (RT-PCR). PMN apoptosis was justified by morphological changes. 
      The cognate antigen(s) of anti-dsDNA on the PMN surface was identified by 
      membrane biotinylation, immunoprecipitation and Western blot. RESULTS: The 
      binding of PMN with anti-dsDNA was much higher than with non-specific mouse IgG2a 
      (70.8 vs 2.0%). Anti-dsDNA at concentrations higher than 12.5 ng/ml significantly 
      enhanced the production and mRNA expression of IL-8 by PMN. However, anti-dsDNA 
      facilitated PMN apoptosis after 3 h incubation. Western blot analysis of 
      biotinylated PMN cell lysates demonstrated that a 50-52 kDa membrane molecule is 
      the cognate antigen of anti-dsDNA. CONCLUSIONS: Anti-dsDNA autoantibody 
      up-regulates IL-8 gene expression and elicits activation-induced cell death 
      (AICD) of human PMN via binding to a 50-52 kDa membrane-expressed molecule.
FAU - Hsieh, S C
AU  - Hsieh SC
AD  - Institute of Clinical Medicine, National Yang-Ming University School of Medicine, 
      Taipei, Taiwan.
FAU - Sun, K H
AU  - Sun KH
FAU - Tsai, C Y
AU  - Tsai CY
FAU - Tsai, Y Y
AU  - Tsai YY
FAU - Tsai, S T
AU  - Tsai ST
FAU - Huang, D F
AU  - Huang DF
FAU - Han, S H
AU  - Han SH
FAU - Yu, H S
AU  - Yu HS
FAU - Yu, C L
AU  - Yu CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carrier Proteins)
RN  - 0 (Interleukin-8)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/*physiology
MH  - Antibodies, Monoclonal/*immunology
MH  - *Apoptosis
MH  - Carrier Proteins/*physiology
MH  - DNA/*immunology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interleukin-8/*genetics
MH  - Mice
MH  - Neutrophils/*physiology
MH  - Up-Regulation
EDAT- 2001/08/21 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/08/21 10:00
PHST- 2001/08/21 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/08/21 10:00 [entrez]
AID - 10.1093/rheumatology/40.8.851 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2001 Aug;40(8):851-8. doi: 10.1093/rheumatology/40.8.851.