PMID- 11513296
OWN - NLM
STAT- MEDLINE
DCOM- 20020207
LR  - 20190901
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 110
IP  - 3
DP  - 2001 Jul
TI  - Heterochromatin and interstitial telomeric DNA homology.
PG  - 214-20
AB  - The purpose of this investigation was twofold. The first objective was to 
      demonstrate that, in most of ten mammalian species commonly used in biomedical 
      research, not all constitutive heterochromatin (C-bands) represents telomeric 
      DNA. For example, the C-bands in human chromosomes, the long arm of the X and the 
      entire Y chromosome of Chinese hamster, and most of the short arms of Peromyscus 
      and Syrian hamster chromosomes are not telomeric DNA. In addition to the usual 
      terminal telomeric DNA in the chromosomes of these mammalian species, the 
      pericentromeric regions of seven or eight Syrian hamster chromosomes and all 
      Chinese hamster chromosomes except pair one have pericentromeric regions that 
      hybridize with telomeric DNA, some in C-bands and some not. The second objective 
      was to describe a simple fluorescence in situ hybridization (FISH) 
      reverse-printing procedure to produce black-and-white microphotographs of 
      metaphase and interphase cells showing locations of telomeric DNA with no loss of 
      resolution. Thus, at least three different types of heterochromatin (telomeric 
      heterochromatin, nontelomeric heterochromatin and a combination of both) are 
      present in these mammalian species, and this simple black-and-white reverse 
      printing of telomeric FISH preparations can depict them economically without 
      sacrificing clarity.
FAU - Multani, A S
AU  - Multani AS
AD  - Department of Cancer Biology, The University of Texas, M.D. Anderson Cancer 
      Center, Houston 77030, USA.
FAU - Ozen, M
AU  - Ozen M
FAU - Furlong, C L
AU  - Furlong CL
FAU - Zhao, Y J
AU  - Zhao YJ
FAU - Hsu, T C
AU  - Hsu TC
FAU - Pathak, S
AU  - Pathak S
LA  - eng
GR  - RRO-4999-01/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Heterochromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Centromere/chemistry
MH  - Chromosome Banding
MH  - DNA/*chemistry/genetics
MH  - Diploidy
MH  - Female
MH  - Heterochromatin/*chemistry/genetics
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Skin/cytology
MH  - Species Specificity
MH  - Telomere/*chemistry
MH  - Y Chromosome/chemistry
EDAT- 2001/08/22 10:00
MHDA- 2002/02/08 10:01
CRDT- 2001/08/22 10:00
PHST- 2001/08/22 10:00 [pubmed]
PHST- 2002/02/08 10:01 [medline]
PHST- 2001/08/22 10:00 [entrez]
AID - 10.1007/s004120100133 [doi]
PST - ppublish
SO  - Chromosoma. 2001 Jul;110(3):214-20. doi: 10.1007/s004120100133.