PMID- 11514352
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 65
IP  - 3
DP  - 2001 Sep
TI  - Ovarian estrogen receptor-beta (ERbeta) regulation: I. Changes in ERbeta 
      messenger RNA expression prior to ovulation in the ewe.
PG  - 866-72
AB  - Ovarian growth and development are critically dependent upon the influence of 
      endogenous estrogens, and both are highly regulated during the reproductive 
      cycle. The observation that estrogen-receptor-alpha-deficient mice still exhibit 
      follicular growth and development, together with other evidence, suggests that 
      responsiveness of the ovary to estradiol occurs predominantly through the second 
      estrogen receptor, ERbeta. We characterized the physiological regulation of 
      ERbeta expression in ovarian follicles during the follicular phase of sheep that 
      were synchronized for estrus during the breeding season with intravaginal 
      progesterone implants (controlled internal drug release [CIDR] device; InterAg, 
      Hamilton, New Zealand). Ovaries were removed at times corresponding to the early 
      (EF) and late follicular phases (LF) of the ovine estrous cycle (12 h [n = 5] and 
      32 h [n = 5] after CIDR device removal, respectively). Sections of ovary were 
      then hybridized with a cRNA probe corresponding to the 5' region of ovine ERbeta. 
      ERbeta mRNA expression within the granulosa layer of different size follicles 
      (size classes: < or =3 mm, 3.1-4.0 mm, 4.1-5.0 mm, >5 mm) was quantified. ERbeta 
      mRNA expression varied both with follicle size (P < 0.01) and with cycle stage (P 
      < 0.01). In EF ewes, the highest levels of ERbeta mRNA expression were found in 
      follicles < or = 3 mm in size. ERbeta mRNA expression declined progressively 
      thereafter among the different size classes with lowest levels expressed in >5-mm 
      follicles. By contrast, expression of ERbeta mRNA in the 3.1- to 4.0-mm follicles 
      of LF group was significantly higher than in the < or =3-mm size follicles and 
      declined thereafter progressively to the >5-mm size levels as in the EF group. 
      Furthermore, expression of ERbeta mRNA in < or =3-mm size follicles of LF group 
      was significantly lower than the corresponding size class in the EF group. Lower 
      expression of ERbeta mRNA in >5-mm follicle is suggestive of a down-regulation by 
      the local estrogen milieu.
FAU - Jansen, H T
AU  - Jansen HT
AD  - Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati, 
      Cincinnati, OH 45267, USA. heiko@vetmed.wsu.edu
FAU - West, C
AU  - West C
FAU - Lehman, M N
AU  - Lehman MN
FAU - Padmanabhan, V
AU  - Padmanabhan V
LA  - eng
GR  - HD23812/HD/NICHD NIH HHS/United States
GR  - P30-HD18528/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (DNA, Complementary)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/chemistry
MH  - Estrogen Receptor beta
MH  - Female
MH  - Follicular Phase
MH  - *Gene Expression Regulation
MH  - Humans
MH  - In Situ Hybridization
MH  - Luteinizing Hormone/metabolism
MH  - Molecular Sequence Data
MH  - *Ovulation
MH  - RNA, Messenger/*analysis
MH  - Receptors, Estrogen/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sheep/*physiology
EDAT- 2001/08/22 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/22 10:00
PHST- 2001/08/22 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/22 10:00 [entrez]
AID - 10.1095/biolreprod65.3.866 [doi]
PST - ppublish
SO  - Biol Reprod. 2001 Sep;65(3):866-72. doi: 10.1095/biolreprod65.3.866.