PMID- 11516017
OWN - NLM
STAT- MEDLINE
DCOM- 20011226
LR  - 20191105
IS  - 0167-6369 (Print)
IS  - 0167-6369 (Linking)
VI  - 70
IP  - 1-2
DP  - 2001 Jul
TI  - Activity monitoring for nitrifying bacteria by fluorescence in situ hybridization 
      and respirometry.
PG  - 223-31
AB  - The activities of two families of nitrifying bacteria were measured by applied 
      respirometer and fluorescence in situ hybridization (FISH) for the purpose of 
      monitoring nitrogen removal. Oxygen uptake rates (OUR) for Nitrosomonas and 
      Nitrobacter were separately measured with the dosing of selective inhibitors 
      during respirometric monitoring. The FISH method was applied to analyze 
      microorganism communities. In 5 mg NH4+-N/L solution, the total nitrogen oxygen 
      demand (NOD) was found to be 6,672 mg DO/L-day with ammonia and nitrite oxidation 
      rate of 4,512 and 2,160 mg DO/L-day, respectively. The oxygen consumption by 
      endogenous oxidation was 288 mg DO/L-day and the biological oxygen demand 936 mg 
      DO/L-day. Respirometric measurements were well matched by the FISH result, which 
      described the presence and quantity of each nitrifying bacteria as nitrification 
      proceeded. It was found that when coupled with OUR data, FISH could provide 
      valuable information about the nitrogen removal process in many practical 
      wastewater treatment systems.
FAU - Kim, I S
AU  - Kim IS
AD  - Department of Environmental Science & Engineering, Kwangju Institute of Science 
      and Technology, Korea. iskim@kjist.ac.kr
FAU - Kim, S Y
AU  - Kim SY
FAU - Jang, A M
AU  - Jang AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Environ Monit Assess
JT  - Environmental monitoring and assessment
JID - 8508350
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/analysis/chemistry
MH  - *Energy Metabolism
MH  - Environmental Monitoring/methods
MH  - *In Situ Hybridization, Fluorescence
MH  - Nitrobacter/*metabolism
MH  - Nitrogen/*metabolism
MH  - Nitrosomonas/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen/*metabolism
MH  - Waste Disposal, Fluid
EDAT- 2001/08/23 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/23 10:00
PHST- 2001/08/23 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/23 10:00 [entrez]
AID - 10.1023/a:1010623919352 [doi]
PST - ppublish
SO  - Environ Monit Assess. 2001 Jul;70(1-2):223-31. doi: 10.1023/a:1010623919352.