PMID- 11518036
OWN - NLM
STAT- MEDLINE
DCOM- 20020108
LR  - 20201208
IS  - 0172-8172 (Print)
IS  - 0172-8172 (Linking)
VI  - 20
IP  - 5
DP  - 2001 Jul
TI  - Infection of synoviocytes with HTLV-I induces telomerase activity.
PG  - 175-9
AB  - To investigate the mechanism of synovial hyperplasia by human T-lymphotropic 
      virus type I (HTLV-I) infection, the enzymatic activity of telomerase and 
      expression of telomerase-related factors in HTLV-I infected synoviocytes were 
      examined. Cultured synoviocytes obtained from four patients with osteoarthritis 
      (OA) and four with traumatic joint disease (TJD) were infected by HTLV-I. 
      Telomerase activity was detected by telomeric repeat amplification protocol 
      (TRAP) assay. Expression of telomerase-related mRNAs such as telomerase reverse 
      transcriptase (hTERT), telomerase RNA component (hTERC), and telomeric repeat 
      binding factor 2 (TRF2) were also examined. Telomerase activity was detected in 
      all HTLV-I-infected synoviocytes but not in uninfected synoviocytes. A remarkable 
      induction of hTERT mRNA was observed in four of eight HTLV-I-infected 
      synoviocytes, whereas expressions of hTERC, TRF2, and TEP-1 mRNAs were not 
      changed. Our results clearly demonstrate that HTLV-I upregulates telomerase 
      activity in synoviocytes probably via upregulation of hTERT activity. These 
      findings suggest that telomerase activation in synoviocytes has an important role 
      in upregulated proliferative activity of HAAP synoviocytes.
FAU - Tsumuki, H
AU  - Tsumuki H
AD  - Rheumatology, Immunology, and Genetics Program, Institute of Medical Science, St 
      Marianna University School of Medicine, Kawasaki, Japan.
FAU - Nakazawa, M
AU  - Nakazawa M
FAU - Hasunuma, T
AU  - Hasunuma T
FAU - Kobata, T
AU  - Kobata T
FAU - Kato, T
AU  - Kato T
FAU - Uchida, A
AU  - Uchida A
FAU - Nishioka, K
AU  - Nishioka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Untranslated)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (TEP1 protein, human)
RN  - 0 (Telomeric Repeat Binding Protein 2)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Carrier Proteins/biosynthesis/genetics
MH  - Cell Division
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - DNA Primers/chemistry
MH  - DNA-Binding Proteins/biosynthesis/genetics
MH  - Human T-lymphotropic virus 1/*physiology
MH  - Humans
MH  - Osteoarthritis, Knee/pathology
MH  - RNA
MH  - RNA, Long Noncoding
MH  - RNA, Messenger/metabolism
MH  - RNA, Untranslated/biosynthesis/genetics
MH  - RNA-Binding Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/enzymology/*virology
MH  - Telomerase/*biosynthesis/genetics
MH  - Telomeric Repeat Binding Protein 2
MH  - Up-Regulation
EDAT- 2001/08/24 10:00
MHDA- 2002/01/10 10:01
CRDT- 2001/08/24 10:00
PHST- 2001/08/24 10:00 [pubmed]
PHST- 2002/01/10 10:01 [medline]
PHST- 2001/08/24 10:00 [entrez]
AID - 10.1007/s002960100111 [doi]
PST - ppublish
SO  - Rheumatol Int. 2001 Jul;20(5):175-9. doi: 10.1007/s002960100111.