PMID- 11518615
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 166
IP  - 1-2
DP  - 2001 Sep 14
TI  - Development of an allergy test model: activation of human mast cells with 
      potentially allergenic substances.
PG  - 91-6
AB  - Due to the permanent increase of newly developed and already existing allergies, 
      simple, quick, and reliable test models for detecting potentially allergenic 
      substances are still required. Here, we describe the development of a new in 
      vitro allergy test based on isolated primary mast cells (MC) of non-allergic 
      patients from lung tissue and foreskin specimens, respectively. To establish the 
      specificity of the test model we used primary MC stimulated with immunoglobulin E 
      (IgE), human recombinant stem cell factor (hrSCF), and anti-IgE antibodies to 
      release significant amounts of histamine indicating the ability of MC to cause a 
      hypersensitivity reaction of the immediate type. The general applicability of 
      this test model for detecting allergenic substances could be confirmed by 
      histamine release of primary MC stimulated with sera of patients suffering from 
      house dust allergy, and the corresponding antigen Dermatophagoides pteronyssinus. 
      The results of the present work suggest that this newly developed human in vitro 
      model provides the opportunity of testing substances for their allergenic 
      potential within days and at low costs. This could also be of particular interest 
      for newly produced compounds.
FAU - Gospos, A
AU  - Gospos A
AD  - Department of Toxicology, Hamburg University and Fraunhofer Institute of 
      Toxicology and Environmental Medicine, ATU, Vogt-Kolln-Str. 30, D-22527 Hamburg, 
      Germany.
FAU - Dreikhausen, U
AU  - Dreikhausen U
FAU - Dartsch, D C
AU  - Dartsch DC
FAU - Szamel, M
AU  - Szamel M
FAU - Hockertz, S
AU  - Hockertz S
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Antibody Formation
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Histamine/*metabolism
MH  - Humans
MH  - Hypersensitivity/*diagnosis/immunology
MH  - Immunoglobulin E/*immunology
MH  - Mast Cells/*immunology/metabolism
MH  - Models, Biological
EDAT- 2001/08/24 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/08/24 10:00
PHST- 2001/08/24 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/24 10:00 [entrez]
AID - S0300-483X(01)00444-9 [pii]
AID - 10.1016/s0300-483x(01)00444-9 [doi]
PST - ppublish
SO  - Toxicology. 2001 Sep 14;166(1-2):91-6. doi: 10.1016/s0300-483x(01)00444-9.