PMID- 11519801
OWN - NLM
STAT- MEDLINE
DCOM- 20020116
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 925
IP  - 1-2
DP  - 2001 Aug 3
TI  - Robotic automated clean-up for detection of fumonisins B1 and B2 in corn and 
      corn-based feed by high-performance liquid chromatography.
PG  - 151-7
AB  - A high-performance liquid chromatography (HPLC) system with fluorescence 
      detection and an automated on-line solid-phase extraction procedure for 
      fumonisins B1 and B2 in corn and corn-based products is described. Different 
      amounts of strong anion-exchange, C18 and end-capped C18 (C(18 ec)) silicas were 
      tested for sample clean-up. Various HPLC parameters were analyzed. The best 
      methodology was found to be extraction with acetonitrile-water and clean up on 
      C(18 ec) disposable extraction cartridges. The system has the advantage of 
      running in an unattended mode of operation and allows processing of 40 samples 
      without system refuel, performing clean-up, o-phthaldialdehyde derivatization, 
      injection and fumonisin detection by fluorescence detection linked to a computer 
      integrator for automated data processing. Recoveries were performed with corn and 
      corn-based feed samples (n=3) spiked with 0.1, 0.5, 1.0, 5.0 and 10 microg/g. 
      Average recoveries for corn and corn-based feed were, respectively, 92.6 and 
      88.3% with relative standard deviations (RSDs) of 5.04 and 6.22%, for fumonisin 
      B1 and 91.2 and 89.0% with RSDs of 5.84 and 7.88% for fumonisin B2. Detection 
      limits (S/N=3) for corn and corn-based feed were approximately 0.03 microg/g for 
      fumonisin B1 and 0.05 microg/g for fumonisin B2
FAU - Dilkin, P
AU  - Dilkin P
AD  - Departamento de Medicina Veterinaria Preventiva-Universidade Federal de Santa 
      Maria, RS, Brazil.
FAU - Mallmann, C A
AU  - Mallmann CA
FAU - de Almeida, C A
AU  - de Almeida CA
FAU - Correa, B
AU  - Correa B
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Carboxylic Acids)
RN  - 0 (Fumonisins)
RN  - 116355-84-1 (fumonisin B2)
RN  - 3ZZM97XZ32 (fumonisin B1)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Carboxylic Acids/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Fumonisins
MH  - Quality Control
MH  - *Robotics
MH  - Zea mays/*chemistry
EDAT- 2001/08/25 10:00
MHDA- 2002/01/17 10:01
CRDT- 2001/08/25 10:00
PHST- 2001/08/25 10:00 [pubmed]
PHST- 2002/01/17 10:01 [medline]
PHST- 2001/08/25 10:00 [entrez]
AID - S0021-9673(01)01037-8 [pii]
AID - 10.1016/s0021-9673(01)01037-8 [doi]
PST - ppublish
SO  - J Chromatogr A. 2001 Aug 3;925(1-2):151-7. doi: 10.1016/s0021-9673(01)01037-8.