PMID- 11520559
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 129
IP  - 1
DP  - 2001 Aug
TI  - Chromosomal aberrations in neuroblastoma cell lines identified by cross species 
      color banding and chromosome painting.
PG  - 10-6
AB  - We have studied cytogenetic rearrangements in karyotypes of five neuroblastoma 
      cell lines [SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, SMS-KCNR] by G-banding, cross 
      species color banding (RxFISH), and fluorescence in situ hybridization (FISH) 
      with chromosome painting probes. Each neuroblastoma cell line had unique modal 
      karyotypic characteristics and showed a variable number of numerical and 
      structural clonal cytogenetic aberrations. The number of rearranged chromosomes 
      in SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, and SMS-KCNR was 11, 3, 7, 14 (tetraploid, 
      20-21), and 6, respectively. The origins of abnormal chromosomes were effectively 
      analyzed by RxFISH and FISH with multiple chromosome painting probes. The 
      chromosomal origin of the homogeneously staining region in SH-SY5Y was identified 
      as coamplification of chromosome bands 2p13 and 2p24 by chromosome 
      microdissection and FISH. The non-random rearrangements of chromosomes were 
      determined on 1p34 approximately p36, 6q16 approximately q21, 8q24, 9q34, 11q13 
      approximately q23, 16q23 approximately q24, 17q21, and 22q31. These results may 
      provide useful information for further molecular characterization of 
      neuroblastoma.
FAU - Kim, G J
AU  - Kim GJ
AD  - Graduate School of Biotechnology, Korea University College of Medicine, 126-1, 
      Anam-Dong 5-Ka, Sungbuk-Ku, Seoul 136-705, South Korea.
FAU - Park, S Y
AU  - Park SY
FAU - Kim, H
AU  - Kim H
FAU - Chun, Y H
AU  - Chun YH
FAU - Park, S H
AU  - Park SH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
CIN - Cancer Genet Cytogenet. 2002 Jun;135(2):196. PMID: 12127407
CIN - Cancer Genet Cytogenet. 2002 Oct 15;138(2):177-8. PMID: 12505268
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - *Chromosome Painting
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neuroblastoma/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2001/08/25 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/25 10:00
PHST- 2001/08/25 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/25 10:00 [entrez]
AID - S0165-4608(01)00418-6 [pii]
AID - 10.1016/s0165-4608(01)00418-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Aug;129(1):10-6. doi: 10.1016/s0165-4608(01)00418-6.