PMID- 11520572
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 129
IP  - 1
DP  - 2001 Aug
TI  - Detection of translocations involving the HOX11/TCL3-locus in 10q24 by interphase 
      fluorescence in situ hybridization.
PG  - 80-4
AB  - The t(10;14)(q24;q11) and its variant t(7;10)(q35;q24), which are recurrent in 
      acute T-cell leukemia, lead to activation of the HOX11/TCL3-gene in chromosomal 
      region 10q24 by juxtaposing this gene to one of the T-cell receptor loci. In the 
      present study, we established a diagnostic assay for detecting these 
      translocations by interphase fluorescence in situ hybridization (FISH). BAC 
      clones flanking the HOX11/TCL3-locus were obtained from a fingerprinted 
      BAC-contig of chromosomal region 10q24. BAC clones located proximal and distal of 
      the HOX11/TCL3-locus were differently labeled and applied to interphase-FISH in 
      seven normal controls and eight T-cell neoplasms with t(10;14)(q24;q11) or 
      t(7;10)(q35;q24). In over 1600 nuclei of controls, a considerable split defined 
      as separation of each one signal for the proximal and distal probe by more than 
      three times the signal diameter was observed in only one cell. In contrast, all 
      T-cell neoplasms with t(10;14) or t(7;10) contained at least 47% of nuclei with a 
      signal split indicating a breakpoint in the HOX11/TCL3-locus. Thus, the 
      established double-color FISH approach provides a new reliable and routinely 
      applicable tool for diagnosing breakpoints in the HOX11/TCL3-locus.
FAU - Kahl, C
AU  - Kahl C
AD  - Institute of Human Genetics, University Hospital Kiel, Schwanenweg 24, D-24105 
      Kiel, Germany.
FAU - Gesk, S
AU  - Gesk S
FAU - Harder, L
AU  - Harder L
FAU - Harbott, J
AU  - Harbott J
FAU - French, L
AU  - French L
FAU - Deloukas, P
AU  - Deloukas P
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Siebert, R
AU  - Siebert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 143275-75-6 (TLX1 protein, human)
SB  - IM
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 10
MH  - Female
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia-Lymphoma, Adult T-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins
MH  - *Translocation, Genetic
EDAT- 2001/08/25 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/25 10:00
PHST- 2001/08/25 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/25 10:00 [entrez]
AID - S0165-4608(01)00426-5 [pii]
AID - 10.1016/s0165-4608(01)00426-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Aug;129(1):80-4. doi: 10.1016/s0165-4608(01)00426-5.