PMID- 11520801
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20220228
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 98
IP  - 5
DP  - 2001 Sep 1
TI  - In vitro spontaneous lymphoproliferation in patients with human T-cell 
      lymphotropic virus type I-associated neurologic disease: predominant expansion of 
      CD8+ T cells.
PG  - 1506-11
AB  - Peripheral blood mononuclear cells (PBMCs) from patients with human T-cell 
      lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic 
      paraparesis (HAM/TSP) proliferate spontaneously in vitro. This spontaneous 
      lymphoproliferation (SP) is one of the immunologic hallmarks of HAM/TSP and is 
      considered to be an important factor related to the pathogenesis of HAM/TSP. 
      However, the cell populations involved in this phenomenon have not yet been 
      definitively identified. To address this issue, the study directly evaluated 
      proliferating cell subsets in SP with a flow cytometric method using 
      bromodeoxyuridine and Ki-67. Although both CD4+ and CD8+ T cells proliferated 
      spontaneously, the percentage of proliferating CD8+ T cells was 2 to 5 times 
      higher than that of CD4+ T cells. In addition, more than 40% of HTLV-I 
      Tax11-19-specific CD8+ T cells as detected by an HLA-A*0201/Tax11-19 tetramer 
      proliferated in culture. In spite of this expansion of HTLV-I-specific CD8+ T 
      cells, HTLV-I proviral load did not decrease. This finding will help elucidate 
      the dynamics of in vivo virus-host immunologic interactions that permit the 
      coexistence of high HTLV-I-specific CD8+ cytotoxic T-lymphocyte responses and 
      high HTLV-I proviral load in HAM/TSP.
FAU - Sakai, J A
AU  - Sakai JA
AD  - Viral Immunology Section, Neuroimmunology Branch, National Institute of 
      Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 
      USA.
FAU - Nagai, M
AU  - Nagai M
FAU - Brennan, M B
AU  - Brennan MB
FAU - Mora, C A
AU  - Mora CA
FAU - Jacobson, S
AU  - Jacobson S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/pathology
MH  - CD8-Positive T-Lymphocytes/immunology/*pathology
MH  - Cell Division
MH  - Cells, Cultured
MH  - DNA Replication
MH  - DNA, Viral/isolation & purification
MH  - Female
MH  - Gene Products, tax/immunology
MH  - HLA Antigens/analysis
MH  - Human T-lymphotropic virus 1/immunology
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Lymphoproliferative Disorders/*etiology/pathology
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/*complications
MH  - Polymerase Chain Reaction
MH  - Proviruses/isolation & purification
EDAT- 2001/08/25 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/08/25 10:00
PHST- 2001/08/25 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/25 10:00 [entrez]
AID - S0006-4971(20)61424-0 [pii]
AID - 10.1182/blood.v98.5.1506 [doi]
PST - ppublish
SO  - Blood. 2001 Sep 1;98(5):1506-11. doi: 10.1182/blood.v98.5.1506.