PMID- 11522008
OWN - NLM
STAT- MEDLINE
DCOM- 20020802
LR  - 20071114
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 86
IP  - 2
DP  - 2001 Aug
TI  - Beta2-glycoprotein I-dependent anticardiolipin antibodies preferentially bind the 
      amino terminal domain of beta2-glycoprotein I.
PG  - 590-5
AB  - Many of the autoantibodies in antiphospholipid syndrome (APS) are directed 
      against beta2-glycoprotein I (beta2-GPI). Recent studies from our laboratories 
      have indicated that the immunodominant binding epitope(s) for high titer, 
      affinity purified antibodies from 11 APS patients are localized to the amino 
      terminal domain (domain 1) of beta2-GPI. The present study employed surface 
      plasmon resonance to localize the immunodominant domain in serum samples from a 
      large cohort of patients with GPL values ranging from 21 to 230 units (n = 106 
      patients). Eighty-eight percent of patients showed > or = threefold selectivity 
      for beta2-GPI containing domain 1 relative to the domain deletion mutant that 
      lacked domain 1. The domain 1 binding activity in patient serum was abolished by 
      removing the IgG fraction from the serum and the binding activity could be fully 
      reconstituted with the IgG fraction. Thus, analysis of serum samples from a large 
      cohort of APS patients indicates that the immunodominant binding epitope(s) for 
      anti-beta2 antibodies are localized to the amino terminal domain of beta2-GPI.
FAU - McNeeley, P A
AU  - McNeeley PA
AD  - La Jolla Pharmaceutical Company, San Diego, CA 92121, USA.
FAU - Dlott, J S
AU  - Dlott JS
FAU - Furie, R A
AU  - Furie RA
FAU - Jack, R M
AU  - Jack RM
FAU - Ortel, T L
AU  - Ortel TL
FAU - Triplett, D A
AU  - Triplett DA
FAU - Victoria, E J
AU  - Victoria EJ
FAU - Linnik, M D
AU  - Linnik MD
LA  - eng
GR  - 1 K24 AI01603-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Antibodies, Anticardiolipin)
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Adult
MH  - Antibodies, Anticardiolipin/*immunology/metabolism
MH  - Antibody Affinity
MH  - Antibody Specificity
MH  - Antiphospholipid Syndrome/immunology
MH  - Autoantibodies/analysis/immunology
MH  - Case-Control Studies
MH  - Cohort Studies
MH  - Epitopes/analysis/chemistry/metabolism
MH  - Female
MH  - Glycoproteins/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin G/analysis/immunology
MH  - Male
MH  - Middle Aged
MH  - Protein Structure, Tertiary
MH  - Surface Plasmon Resonance
MH  - beta 2-Glycoprotein I
EDAT- 2001/08/28 10:00
MHDA- 2002/08/03 10:01
CRDT- 2001/08/28 10:00
PHST- 2001/08/28 10:00 [pubmed]
PHST- 2002/08/03 10:01 [medline]
PHST- 2001/08/28 10:00 [entrez]
AID - 01080590 [pii]
PST - ppublish
SO  - Thromb Haemost. 2001 Aug;86(2):590-5.