PMID- 11522858
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190513
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 16
IP  - 9
DP  - 2001 Sep
TI  - Cultured rat glomerular epithelial cells show gene expression and production of 
      transforming growth factor-beta: expression is enhanced by thrombin.
PG  - 1776-82
AB  - BACKGROUND: Glomerular crescents play an important role in progressive glomerular 
      injury. The lesions consist of epithelial cells, macrophages, and deposits of 
      fibrin and extracellular matrix. Transforming growth factor beta (TGF-beta) 
      contributes to the modulation of cell growth and extracellular matrix synthesis. 
      Thrombin is involved in fibrin formation in crescents. The purpose of this study 
      was to examine whether glomerular epithelial cells (GEC) could produce TGF-beta, 
      and if so, to clarify the role of TGF-beta in GEC proliferation. We also 
      investigated whether thrombin could modulate the production of TGF-beta and 
      extracellular matrix by GEC. METHODS: Bioassay using the TGF-beta-dependent mink 
      pulmonary epithelial cell line (CCL-64), immunoblot analysis, and reverse 
      transcriptase polymerase chain reaction (RT-PCR) were used to demonstrate 
      TGF-beta production by rat GEC. TGF-beta gene expression was examined by RT-PCR 
      in GEC incubated with thrombin, and type IV collagen and fibronectin were 
      quantified by enzyme immunoassay in culture supernatants of GEC incubated with 
      thrombin or TGF-beta. RESULTS: TGF-beta activity was demonstrated in GEC 
      supernatants by bioassay. Immunoblot analysis of concentrated culture 
      supernatants using anti-TGF-beta antibody revealed a 12.5-kDa protein, which was 
      compatible with TGF-beta. Concentrated GEC supernatants inhibited GEC 
      proliferation as well as porcine TGF-beta. RT-PCR demonstrated TGF-beta gene 
      expression in GEC. Thrombin (0.5-5.0 U/ml) enhanced TGF-beta mRNA expression in a 
      dose-dependent manner. Thrombin (5.0 U/ml) and porcine TGF-beta (5.0 ng/ml) 
      stimulated the production of type IV collagen and fibronectin by GEC. 
      CONCLUSIONS: Rat GEC produce TGF-beta in vitro. Thrombin may participate in the 
      progression of glomerulosclerosis in crescentic glomerulonephritis through the 
      stimulation of TGF-beta production by GEC.
FAU - Tsunoda, S
AU  - Tsunoda S
AD  - Second Department of Internal Medicine, Hirosaki University School of Medicine, 
      Zaifu-cho 5, Hirosaki 036-8216, Japan.
FAU - Yamabe, H
AU  - Yamabe H
FAU - Osawa, H
AU  - Osawa H
FAU - Kaizuka, M
AU  - Kaizuka M
FAU - Shirato, K
AU  - Shirato K
FAU - Okumura, K
AU  - Okumura K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Collagen Type IV)
RN  - 0 (Fibronectins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Collagen Type IV/biosynthesis
MH  - Epithelial Cells/cytology/physiology
MH  - Fibronectins/biosynthesis
MH  - *Gene Expression/drug effects
MH  - Kidney Glomerulus/cytology/*physiology
MH  - Rats
MH  - Swine
MH  - Thrombin/*pharmacology
MH  - Transforming Growth Factor beta/*biosynthesis/*genetics
EDAT- 2001/08/28 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/28 10:00
PHST- 2001/08/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/28 10:00 [entrez]
AID - 10.1093/ndt/16.9.1776 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2001 Sep;16(9):1776-82. doi: 10.1093/ndt/16.9.1776.