PMID- 11524234
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 170
IP  - 3
DP  - 2001 Sep
TI  - Effect of diethylstilbestrol on cell proliferation and expression of epidermal 
      growth factor in the developing female rat reproductive tract.
PG  - 539-54
AB  - To evaluate mechanisms of cell proliferation in the fetal female rat reproductive 
      tract, diethylstilbestrol (DES) effects on cell division and estrogen receptor 
      (ER), epidermal growth factor (EGF) and EGF receptor (EGF-R) expressions were 
      determined from gestational day (GD) 15.5 to 21.5. Reproductive tracts were 
      evaluated within three regions along the Mullerian duct axis; these were 
      proximal, middle and caudal, which differentiate into oviduct, uterus and upper 
      vagina respectively. In fetuses from non-treated dams, epithelial and mesenchymal 
      proliferation, as evaluated by 5-bromo-2'-deoxyuridine incorporation, was 
      decreased with development in all regions of the Mullerian duct. EGF levels were 
      determined by immunohistochemistry. Mullerian epithelial EGF immunoreactivity was 
      intense in the proximal and middle regions on GDs 15.5 and 17.5. EGF staining 
      remained intense only in the proximal epithelia by GD 19.5 and was weak in the 
      caudal epithelium, but substantially reduced throughout epithelia in all regions 
      by GD 21.5. Thus, decreased cell proliferation correlated with decreased EGF 
      expression in the developing Mullerian duct. DES (100 microg/kg body weight) was 
      injected from GD 15 to 19 and female fetuses were collected on GD 19.5. DES 
      increased Mullerian duct cell proliferation in the proximal epithelium and 
      mesenchyme but decreased it in the caudal epithelium compared with oil-treated 
      controls. No proliferative DES effect was observed in any cell type in the middle 
      region. Mullerian duct EGF immunoreactivity was suppressed by DES compared with 
      oil. Competitive RT-PCR indicated DES also decreased mRNAs for EGF, ERbeta1 and 
      ERbeta2, but not ERalpha and EGF-R. These results indicate EGF may be an 
      important regulatory factor of Mullerian duct cell proliferation, and that DES 
      may alter cell proliferation by disrupting normal EGF, ERbeta1 and ERbeta2 
      expression in the developing female rat reproductive tract.
FAU - Okada, A
AU  - Okada A
AD  - Safety Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd, Itabashi, Tokyo 
      174-8511, Japan.
FAU - Sato, T
AU  - Sato T
FAU - Ohta, Y
AU  - Ohta Y
FAU - Buchanan, D L
AU  - Buchanan DL
FAU - Iguchi, T
AU  - Iguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Diethylstilbestrol/*pharmacology
MH  - Embryonic and Fetal Development/*drug effects
MH  - Epidermal Growth Factor/*drug effects/metabolism
MH  - ErbB Receptors/drug effects/metabolism
MH  - Estrogens, Non-Steroidal/*pharmacology
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Mullerian Ducts/cytology/*drug effects/embryology
MH  - Ovary/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Uterus/metabolism
EDAT- 2001/08/29 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/08/29 10:00
PHST- 2001/08/29 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/29 10:00 [entrez]
AID - JOE04276 [pii]
AID - 10.1677/joe.0.1700539 [doi]
PST - ppublish
SO  - J Endocrinol. 2001 Sep;170(3):539-54. doi: 10.1677/joe.0.1700539.