PMID- 11525803
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190826
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 119
IP  - 1
DP  - 2001 Sep 3
TI  - DA rat NK(+)CD3(-) cells inhibit autoreactive T-cell responses.
PG  - 81-7
AB  - We isolated natural killer (NK) cells from DA rat bone marrow (BM) by staining 
      with PE anti-NKR-P1A and FACS sorting (>98% NK(+)). The purified NK cells inhibit 
      T-cell proliferation in a dosage-dependent fashion and suppressed production of 
      the proinflammatory Th1 cytokine, IFN-gamma. When activated in vitro with Con A 
      supernatant (CAS), the purified NK cells secrete the beta-chemokine monocyte 
      chemoattractant protein-1 (MCP-1) and upregulate MCP-1 mRNA. The activated NK 
      cells also express IFN-gamma mRNA. Sorted NK(+)CD3(-) cells, from which NKT cells 
      have been excluded, also inhibit autoreactive T-cell responses to myelin basic 
      protein (MBP). These findings are consistent with a role for conventional NK 
      cells in maintaining immune homeostasis, by eliminating autoreactive T cells that 
      have inadvertently become activated.
FAU - Wolf, N A
AU  - Wolf NA
AD  - Department of Immunology and Microbiology, Wayne State University School of 
      Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA.
FAU - Swanborg, R H
AU  - Swanborg RH
LA  - eng
GR  - 5 RO1 NS06985-34/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (CD3 Complex)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/immunology/physiology
MH  - CD3 Complex/*analysis
MH  - Cell Division/physiology
MH  - Cell Separation
MH  - Chemokine CCL2/genetics/metabolism
MH  - Flow Cytometry
MH  - Interferon-gamma/antagonists & inhibitors/biosynthesis/genetics
MH  - Killer Cells, Natural/*immunology/*physiology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*cytology/*immunology
EDAT- 2001/08/30 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/08/30 10:00
PHST- 2001/08/30 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/30 10:00 [entrez]
AID - S0165-5728(01)00383-6 [pii]
AID - 10.1016/s0165-5728(01)00383-6 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2001 Sep 3;119(1):81-7. doi: 10.1016/s0165-5728(01)00383-6.