PMID- 11525924
OWN - NLM
STAT- MEDLINE
DCOM- 20011218
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 497
IP  - 1-2
DP  - 2001 Oct 18
TI  - Antioxidative and antimutagenic activity of yeast cell wall mannans in vitro.
PG  - 213-22
AB  - Antioxidative and antimutagenic effect of yeast cell wall mannans, in particular, 
      extracellular glucomannan (EC-GM) and glucomannan (GM-C.u.) both from Candida 
      utilis, mannan from Saccharomyces cerevisiae (M-S.c.) and mannan from Candida 
      albicans (M-C.a.) was evaluated. Luminol-dependent photochemical method using 
      trolox as a standard showed that EC-GM, GM-C.u., M-S.c. and M-C.a. have 
      relatively good antioxidative properties. EC-GM exhibited the highest 
      antioxidative activity, followed by GM-C.u. and M-S.c. M-C.a. showed the least 
      antioxidative activity. These mannans were experimentally confirmed to exhibit 
      different, statistically significant antimutagenic activity in reducing damage of 
      chloroplast DNA of the flagellate Euglena gracilis induced by ofloxacin and 
      acridine orange (AO). We suggest that the antimutagenic effect of EC-GM, GM-C.u., 
      M-S.c. and M-C.a. against ofloxacin is based on their ability to scavenge 
      reactive oxygen radicals. With AO, the reduction of the chloroplast DNA lession 
      could be a result of the absorptive capacity of the mannans. The important 
      characteristics of mannans isolated from the yeast cell walls, such as good water 
      solubility, relatively small molecular weight (15-30kDa), and antimutagenic 
      effect exerted through different mode of action, appear to be a promising 
      features for their prospective use as a natural protective (antimutagenic) 
      agents.
FAU - Krizkova, L
AU  - Krizkova L
AD  - Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, 
      Odborarske namestie 5, 811 07, Bratislava, Slovak Republic. 
      krizkova@m2.fedu.uniba.sr
FAU - Durackova, Z
AU  - Durackova Z
FAU - Sandula, J
AU  - Sandula J
FAU - Sasinkova, V
AU  - Sasinkova V
FAU - Krajcovic, J
AU  - Krajcovic J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (DNA, Chloroplast)
RN  - 0 (Mannans)
RN  - 36W3E5TAMG ((1-6)-alpha-glucomannan)
RN  - 5EXP385Q4F (Luminol)
RN  - A4P49JAZ9H (Ofloxacin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/toxicity
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Candida/chemistry
MH  - Candida albicans/chemistry
MH  - Cell Wall/chemistry
MH  - DNA Damage
MH  - DNA, Chloroplast/drug effects
MH  - Euglena gracilis/drug effects
MH  - In Vitro Techniques
MH  - Luminol
MH  - Mannans/isolation & purification/*pharmacology
MH  - Mutagenicity Tests
MH  - Ofloxacin/toxicity
MH  - Photochemistry
MH  - Saccharomyces cerevisiae/chemistry
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2001/08/30 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/30 10:00
PHST- 2001/08/30 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/30 10:00 [entrez]
AID - S1383571801002571 [pii]
AID - 10.1016/s1383-5718(01)00257-1 [doi]
PST - ppublish
SO  - Mutat Res. 2001 Oct 18;497(1-2):213-22. doi: 10.1016/s1383-5718(01)00257-1.