PMID- 11529897
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20190901
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 31
IP  - 8
DP  - 2001 Aug
TI  - IFN-gamma production in response to IL-18 or IL-12 stimulation by peripheral 
      blood mononuclear cells of atopic patients.
PG  - 1263-70
AB  - BACKGROUND: Several studies have shown that interleukin (IL)-4 and 
      interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E 
      (IgE) production and that IL-18 and IL-12 induce IFN-gamma. OBJECTIVE: IFN-gamma 
      production in response to IL-18 or IL-12 stimulation was investigated in 
      peripheral blood mononuclear cells (PBMCs) of atopic patients with various levels 
      of serum IgE. METHODS: Cytokine production from PBMCs was measured following 
      stimulation with a non-specific stimulator (phytohemagglutinin: PHA), IL-18 or 
      IL-12 in 12 healthy controls and 26 atopic patients with various serum IgE 
      levels. RESULTS: IFN-gamma production by IL-18-stimulated PBMCs was positively 
      correlated with IFN-gamma production by IL-12-stimulated PBMCs (P < 0.05). 
      However some atopic patients showed discrepancy between the levels of IFN-gamma 
      production stimulated by IL-12 and by IL-18. CONCLUSIONS: The results shown here 
      suggest the presence of abnormalities in the IL-12 and/or IL-18 signalling 
      pathways, such as genetic defects in the atopic patients.
FAU - Shikano, H
AU  - Shikano H
AD  - Department of Paediatrics, Gifu University School of Medicine, 40 Tsukasa-machi, 
      Gifu 500-8705, Japan. hshikano@cc.gifu-u.ac.jp
FAU - Kato, Z
AU  - Kato Z
FAU - Kaneko, H
AU  - Kaneko H
FAU - Watanabe, M
AU  - Watanabe M
FAU - Inoue, R
AU  - Inoue R
FAU - Kasahara, K
AU  - Kasahara K
FAU - Takemura, M
AU  - Takemura M
FAU - Kondo, N
AU  - Kondo N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Interleukin-18)
RN  - 0 (Phytohemagglutinins)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Infant
MH  - Interferon-gamma/*biosynthesis/immunology
MH  - Interleukin-12/*pharmacology
MH  - Interleukin-18/*pharmacology
MH  - Leukocytes, Mononuclear/*immunology/*metabolism
MH  - Male
MH  - Phytohemagglutinins/immunology
MH  - Time Factors
EDAT- 2001/09/01 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/01 10:00
PHST- 2001/09/01 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/01 10:00 [entrez]
AID - cea1141 [pii]
AID - 10.1046/j.1365-2222.2001.01141.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2001 Aug;31(8):1263-70. doi: 10.1046/j.1365-2222.2001.01141.x.