PMID- 11531103
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20191021
IS  - 0939-5075 (Print)
IS  - 0341-0382 (Linking)
VI  - 56
IP  - 7-8
DP  - 2001 Jul-Aug
TI  - Evaluation of delayed apoptotic response in lethally irradiated human melanoma 
      cell lines.
PG  - 660-5
AB  - To assess the lethal doses of gamma radiation and corresponding apoptotic 
      response in new established human melanoma cell lines we exposed exponentially 
      growing cultures to 8-100 Gy gamma radiation. The apoptosis and cell survival 
      were determined by trypan blue exclusion, terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) reaction, agarose gel 
      electrophoresis, colony forming assay, and long-term survival assay. The maximal 
      DNA fragmentation 3 days after irradiation was observed in cultures irradiated 
      with 20 Gy (36.9% TUNEL positive cells). The cultures irradiated with 50 and 100 
      Gy contained 18.7% and 16.4% TUNEL positive cells, respectively. Cultures exposed 
      to 8 and 20 Gy gamma radiation recovered by week 3-4. Lethally irradiated (50 and 
      100 Gy) cultures which contained less apoptotic cells by day 3 died by week 5. A 
      detectable increase in melanoma cell pigmentation after irradiation was also 
      observed. The survival of human melanoma cell cultures after exposure to gamma 
      radiation does not correlate with the level of apoptotic cells by day 3. At high 
      radiation doses (> 50 Gy) when the radiation induced cell pigmentation is not 
      inhibited the processes of apoptotic DNA fragmentation might be preferentially 
      inactivated.
FAU - Tsoncheva, V L
AU  - Tsoncheva VL
AD  - Department of Cell Biology, Institute of Molecular Biology, Bulgarian Academy of 
      Sciences, Sofia.
FAU - Kirov, K S
AU  - Kirov KS
FAU - Valkova, C A
AU  - Valkova CA
FAU - Milchev, G I
AU  - Milchev GI
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Z Naturforsch C J Biosci
JT  - Zeitschrift fur Naturforschung. C, Journal of biosciences
JID - 8912155
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Apoptosis/physiology/*radiation effects
MH  - Cell Division/radiation effects
MH  - Cell Survival/*radiation effects
MH  - Cisplatin/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Dose-Response Relationship, Radiation
MH  - *Gamma Rays
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Melanoma
MH  - Tumor Cells, Cultured
EDAT- 2001/09/04 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/09/04 10:00
PHST- 2001/09/04 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/09/04 10:00 [entrez]
AID - 10.1515/znc-2001-7-827 [doi]
PST - ppublish
SO  - Z Naturforsch C J Biosci. 2001 Jul-Aug;56(7-8):660-5. doi: 
      10.1515/znc-2001-7-827.