PMID- 11531292
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20061115
IS  - 0090-8258 (Print)
IS  - 0090-8258 (Linking)
VI  - 82
IP  - 2
DP  - 2001 Aug
TI  - High sensitivity of PCR in situ hybridization for the detection of human 
      papillomavirus infection in uterine cervical neoplasias.
PG  - 350-4
AB  - OBJECTIVE: This study was designed in order to examine whether the sensitivity of 
      PCR in situ hybridization (PISH) is superior to that of fluorescence in situ 
      hybridization (FISH) and immunocytochemical staining (ICS) in detecting the 
      presence of human papillomavirus (HPV) infection in uterine cervical neoplasias. 
      METHODS: Three cell specimens were obtained from every 54 patients who had been 
      histologically and cytologically diagnosed as severe dysplasia of the uterine 
      cervix (SD), carcinoma in situ of the uterine cervix (CIS), or microinvasive 
      carcinoma of the uterine cervix (MIC). All of them were first stained by the 
      Papanicolaou (pap) method before the experiments. After specimens were classified 
      into SD (n = 17) and CIS/MIC groups (n = 37), the sensitivities of detection for 
      HPV infection were compared among PISH, FISH, and ICS methods which had been 
      carried out after decolorization of the pap stain. RESULTS: In the SD group, PISH 
      demonstrated a 58.8% positive incidence of HPV, which was significantly higher 
      than that (23.5%) shown by FISH (P < 0.05). There was no significant difference 
      in the positivities of HPV between the PISH and ICS (47.1%) methods. In the 
      CIS/MIC group, PISH, FISH, and ICS showed 73.0, 43.2, and 54.1% positive 
      incidences of HPV, respectively. There was significant difference in HPV 
      positivities between PISH and FISH as well as ICS (P < 0.01, P < 0.05). The total 
      frequency of the detection of HPV infection by PISH was significantly higher than 
      that by FISH (P < 0.01) and high with a marginal significance compared to that by 
      ICS (P = 0.051). Moreover, the morphologic change of a single cell and the 
      HPV-positive signals could be observed simultaneously by the PISH technique. 
      CONCLUSION: Because PISH possesses high sensitivity for the detection of HPV 
      infection in morphologically intact cells from uterine cervical neoplasia, the 
      PISH technique might have promising potential for application to the detection of 
      HPV presence in situ in screening.
CI  - Copyright 2001 Academic Press.
FAU - Xiao, Y
AU  - Xiao Y
AD  - Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, 
      5 Zaifu-cho, Hirosaki, Aomori, 036-8562, Japan.
FAU - Sato, S
AU  - Sato S
FAU - Oguchi, T
AU  - Oguchi T
FAU - Kudo, K
AU  - Kudo K
FAU - Yokoyama, Y
AU  - Yokoyama Y
FAU - Saito, Y
AU  - Saito Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gynecol Oncol
JT  - Gynecologic oncology
JID - 0365304
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Carcinoma/virology
MH  - Carcinoma in Situ/virology
MH  - DNA, Viral/analysis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - Papillomaviridae/*genetics
MH  - Papillomavirus Infections/complications/*diagnosis/virology
MH  - Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Tumor Virus Infections/complications/*diagnosis/virology
MH  - Uterine Cervical Neoplasms/*virology
EDAT- 2001/09/05 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/09/05 10:00
PHST- 2001/09/05 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/09/05 10:00 [entrez]
AID - S0090-8258(01)96261-4 [pii]
AID - 10.1006/gyno.2001.6261 [doi]
PST - ppublish
SO  - Gynecol Oncol. 2001 Aug;82(2):350-4. doi: 10.1006/gyno.2001.6261.