PMID- 11533280
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20211203
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 131
IP  - 9
DP  - 2001 Sep
TI  - Dietary (n-3) polyunsaturated fatty acids exert antihypertensive effects by 
      modulating calcium signaling in T cells of rats.
PG  - 2364-9
AB  - After 10 wk of feeding an experimental diet enriched with (n-3) polyunsaturated 
      fatty acids (PUFA), i.e., eicosapentaenoic acid [EPA, 20:5(n-3)] and [DHA, 
      22:6(n-3)] (EPAX), blood pressure in spontaneously hypertensive rats (SHR), but 
      not in normotensive Wistar-Kyoto (WKY) rats was reduced relative to rats fed an 
      unsupplemented control diet. Concanavalin A-stimulated T-cell proliferation was 
      diminished in both strains of rats fed the PUFA/EPAX diet. The experimental diet 
      lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats 
      fed the control diet. To determine whether there was a defect in calcium 
      homeostasis in T cells during hypertension, we employed the following agents: 
      caffeine, which recruits calcium from the cytosolic Ca(2+)-induced Ca(2+)-release 
      pool; ionomycin, which at low concentrations opens calcium channels; and 
      thapsigargin (TG), which mobilizes [Ca(2+)]i from the endoplasmic reticulum (ER) 
      pool. Caffeine-induced increases in [Ca(2+)]i were not modified by the PUFA/EPAX 
      diet. The ionomycin-induced increases in [Ca(2+)]i in T cells from SHR were 
      greater than in those from WKY rats; consumption of the PUFA/EPAX diet did not 
      modify Ca(2+) influx in cells of either strain. The TG-induced increases in 
      [Ca(2+)]i in T cells from SHR were greater than those in cells from WKY rats. 
      Interestingly, consumption of the experimental diet reduced TG-evoked increases 
      in [Ca(2+)]i in T cells from SHR and increased those in T cells from WKY rats, 
      indicating that the PUFA/EPAX diet could reverse the calcium mobilization from 
      the ER pool in T cells. These results suggest that (n-3) PUFA exert 
      antihypertensive effects and modulate T-cell calcium signaling during 
      hypertension in rats.
FAU - Triboulot, C
AU  - Triboulot C
AD  - UPRES Lipids & Nutrition, Faculty of Life Sciences, Universite de Bourgogne, 
      Dijon 21000, France.
FAU - Hichami, A
AU  - Hichami A
FAU - Denys, A
AU  - Denys A
FAU - Khan, N A
AU  - Khan NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Dietary Fats)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Omega-6)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Phospholipids)
SB  - IM
MH  - Animals
MH  - Antihypertensive Agents/*pharmacology
MH  - Binding, Competitive
MH  - Calcium Signaling/*drug effects
MH  - Dietary Fats/*pharmacology
MH  - Fatty Acids, Omega-3/*pharmacology
MH  - Fatty Acids, Omega-6
MH  - Fatty Acids, Unsaturated/metabolism/*pharmacology
MH  - Immunosuppression Therapy
MH  - Intracellular Membranes/metabolism
MH  - Male
MH  - Phospholipids/metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - T-Lymphocytes/*metabolism
EDAT- 2001/09/05 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/05 10:00
PHST- 2001/09/05 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/05 10:00 [entrez]
AID - 10.1093/jn/131.9.2364 [doi]
PST - ppublish
SO  - J Nutr. 2001 Sep;131(9):2364-9. doi: 10.1093/jn/131.9.2364.