PMID- 11535522
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20220228
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 98
IP  - 6
DP  - 2001 Sep 15
TI  - CD8(+) T cells are an in vivo reservoir for human T-cell lymphotropic virus type 
      I.
PG  - 1858-61
AB  - It is thought that human T-cell lymphotropic virus type I (HTLV-I) preferentially 
      infects CD4(+) T cells in vivo. However, observations of high HTLV-I proviral 
      load in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis 
      suggest that HTLV-I may infect other cell types in addition to CD4(+) T cells. To 
      identify in vivo T-cell tropisms of HTLV-I, real-time quantitative polymerase 
      chain reaction (PCR) and intracellular protein staining were used. A high amount 
      of HTLV-I proviral DNA was detected from purified CD8(+) T cells by quantitative 
      PCR (between 1.64 and 62.83 copies of HTLV-I provirus per 100 isolated CD8(+) T 
      cells). CD8(+) T cells expressed HTLV-I-related antigens (HTLV-I Tax and p19 
      protein) after a short time in cultivation. These results demonstrate that CD8(+) 
      T cells are also infected with HTLV-I and express HTLV-I antigens at levels that 
      are comparable to HTLV-I-infected CD4(+) cells. Therefore, CD8(+) cells are an 
      additional viral reservoir in vivo for HTLV-I and may contribute to the 
      pathogenesis of HTLV-I-mediated disorders.
FAU - Nagai, M
AU  - Nagai M
AD  - Viral Immunology Section, Neuroimmunology Branch, National Institute of 
      Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 
      USA.
FAU - Brennan, M B
AU  - Brennan MB
FAU - Sakai, J A
AU  - Sakai JA
FAU - Mora, C A
AU  - Mora CA
FAU - Jacobson, S
AU  - Jacobson S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (Gene Products, tax)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - 0 (p19 protein, Human T-lymphotropic virus 1)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/virology
MH  - CD8-Positive T-Lymphocytes/*virology
MH  - Cells, Cultured
MH  - DNA, Viral/analysis
MH  - Gene Products, gag/metabolism
MH  - Gene Products, tax/metabolism
MH  - Human T-lymphotropic virus 1/genetics/*isolation & purification
MH  - Humans
MH  - Retroviridae Proteins, Oncogenic/metabolism
MH  - gag Gene Products, Human Immunodeficiency Virus
EDAT- 2001/09/06 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/09/06 10:00
PHST- 2001/09/06 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/09/06 10:00 [entrez]
AID - S0006-4971(20)60949-1 [pii]
AID - 10.1182/blood.v98.6.1858 [doi]
PST - ppublish
SO  - Blood. 2001 Sep 15;98(6):1858-61. doi: 10.1182/blood.v98.6.1858.