PMID- 11535646
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20231213
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 92
IP  - 4
DP  - 2001 Jul-Aug
TI  - Independent spontaneous mitochondrial malate dehydrogenase null mutants in 
      soybean are the result of deletions.
PG  - 333-8
AB  - The mitochondrial malate dehydrogenase-1 (Mdh1) gene of soybean [Glycine max (L.) 
      Merr.] spontaneously mutates to a null phenotype at a relatively high rate. To 
      determine the molecular basis for the instability of the Mdh1 gene, the gene was 
      cloned and sequenced. The null phenotype correlated with the deletion of specific 
      genomic restriction fragments that encode the Mdh1 gene. The composition of the 
      Mdh1 gene and its environs were compared with those of the more stable MDH2 gene. 
      Several possible causes of the observed instability were found, including 
      duplications, repeats, and two regions with similarity to a soybean catalase. The 
      most likely cause of instability, however, appeared to be a 1233 bp region with 
      58.9% identity to the Cyclops retrotransposons. Translation of a 714 bp segment 
      of this region produced a peptide composed of 238 amino acid residues that showed 
      35-40% identity and 55-60% similarity to several putative Cyclops gag-pol 
      proteins (group-specific antigen polyprotein). This short peptide also contained 
      a segment that corresponded to the protease active site of the gag-pol protein. 
      Thus in an appropriate genetic background, a retrotransposon, whether whole or 
      fractured, could promote genetic rearrangements.
FAU - Imsande, J
AU  - Imsande J
AD  - Department of Agronomy, Iowa State University, Ames, Iowa, USA.
FAU - Pittig, J
AU  - Pittig J
FAU - Palmer, R G
AU  - Palmer RG
FAU - Wimmer, C
AU  - Wimmer C
FAU - Gietl, C
AU  - Gietl C
LA  - eng
SI  - GENBANK/AB007467
SI  - GENBANK/AC006250
SI  - GENBANK/AF020273
SI  - GENBANK/AF068686
SI  - GENBANK/AF068687
SI  - GENBANK/AF068688
SI  - GENBANK/AF068689
SI  - GENBANK/AF180335
SI  - GENBANK/AJ000640
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
SB  - IM
MH  - Cloning, Molecular
MH  - *Gene Deletion
MH  - Genes, Plant
MH  - Malate Dehydrogenase/*genetics
MH  - Mitochondria/*enzymology
MH  - Molecular Sequence Data
MH  - Glycine max/*enzymology
EDAT- 2001/09/06 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/09/06 10:00
PHST- 2001/09/06 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/09/06 10:00 [entrez]
AID - 10.1093/jhered/92.4.333 [doi]
PST - ppublish
SO  - J Hered. 2001 Jul-Aug;92(4):333-8. doi: 10.1093/jhered/92.4.333.