PMID- 11536311
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190915
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 48
IP  - 4
DP  - 2001 Sep 15
TI  - Expression of a human cell adhesion molecule, MUC18, in prostate cancer cell 
      lines and tissues.
PG  - 305-15
AB  - BACKGROUND: Over expression of huMUC18, a cell adhesion molecule in the 
      immunoglobulin gene superfamily, causes a non-metastatic human melanoma cell line 
      to become metastatic in a nude mouse system. To determine if MUC18 expression 
      correlates with the malignant progression of prostate cancer, we investigated 
      differential expression of human MUC18 (huMUC18) in normal prostate epithelial 
      cells, prostate cancer cell lines, and prostatic normal and cancer tissues. 
      METHODS: RT-PCR and Western blot analyses were used to analyze the expression of 
      MUC18 mRNA and protein in four human prostate cancer cell lines, cultured primary 
      normal prostate epithelial cells, normal prostate and malignant prostate tissues. 
      Immunohistochemistry was used to determine the expression of MUC18 antigen in 
      prostatic tissues at different stages of malignancy. RESULTS: Human MUC18 mRNA 
      and protein was expressed in three different prostate cancer cell lines (TSU-PR1, 
      DU145, and PC-3), but not in one prostate cancer cell line (LNCaP.FGC). HuMUC18 
      protein was also expressed at high levels in extracts prepared from tissue sample 
      sections containing high grade prostatic intraepithelial neoplasia (PIN), but 
      weakly expressed in extracts prepared from either cultured primary normal 
      prostatic epithelial cells or the normal prostate gland. Immunohistochemical 
      analysis showed that huMUC18 was expressed at higher levels in the epithelial 
      cells of high-grade PIN and prostatic carcinomas and in cells of a lymph node 
      metastasis compared to that in normal or benign hyperplastic epithelium (BPH). 
      CONCLUSIONS: We therefore conclude that MUC18 is expressed at higher levels in 
      pre-malignant and malignant prostatic epithelium, including metastasis. We 
      suggest that over-expression of MUC18 may be a new marker of human prostate 
      cancer and also implicates its possible role in development and progression of 
      prostate cancer.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Wu, G J
AU  - Wu GJ
AD  - Department of Microbiology & Immunology, Emory University School of Medicine, 
      Atlanta, Georgia 30322, USA. wu@microbio.emory.edu
FAU - Varma, V A
AU  - Varma VA
FAU - Wu, M W
AU  - Wu MW
FAU - Wang, S W
AU  - Wang SW
FAU - Qu, P
AU  - Qu P
FAU - Yang, H
AU  - Yang H
FAU - Petros, J A
AU  - Petros JA
FAU - Lim, S D
AU  - Lim SD
FAU - Amin, M B
AU  - Amin MB
LA  - eng
GR  - R21CA69764/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Surface)
RN  - 0 (CD146 Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA, Complementary)
RN  - 0 (MCAM protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Antigens, CD
MH  - Antigens, Surface/*biosynthesis/genetics
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - CD146 Antigen
MH  - Cell Adhesion Molecules/*biosynthesis/genetics
MH  - DNA, Complementary/genetics
MH  - Epithelial Cells/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - *Membrane Glycoproteins
MH  - *Neural Cell Adhesion Molecules
MH  - Precancerous Conditions/metabolism
MH  - Prostate/metabolism
MH  - Prostatic Neoplasms/genetics/*metabolism/pathology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2001/09/06 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/09/06 10:00
PHST- 2001/09/06 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/09/06 10:00 [entrez]
AID - 10.1002/pros.1111 [pii]
AID - 10.1002/pros.1111 [doi]
PST - ppublish
SO  - Prostate. 2001 Sep 15;48(4):305-15. doi: 10.1002/pros.1111.