PMID- 11541509
OWN - NASA
STAT- MEDLINE
DCOM- 19990320
LR  - 20191027
IS  - 1065-7541 (Print)
IS  - 1065-7541 (Linking)
VI  - 3
DP  - 1996
TI  - Radiogenic transformation of human mammary epithelial cells in vitro.
PG  - 412-9
AB  - Cancer induction by space radiations is a major concern for manned space 
      exploration. Accurate assessment of radiation risk at low doses requires basic 
      understanding of mechanism(s) of radiation carcinogenesis. For determining the 
      oncogenic effects of ionizing radiation in human epithelial cells, we transformed 
      a mammary epithelial cell line (185B5), which was immortalized by benzo(a)pyrene, 
      with energetic heavy ions and obtained several transformed clones. These 
      transformed cells showed growth properties on Matrigel similar to human mammary 
      tumor cells. To better understand the mechanisms of radiogenic transformation of 
      human cells, we systematically examined the alterations in chromosomes and cancer 
      genes. Among 16 autosomes examined for translocations, by using fluorescence in 
      situ hybridization (FISH) technique, chromosomes 3, 12, 13, 15, 16, and 18 
      appeared to be normal in transformed cells. Chromosomes 1, 4, 6, 8, and 17 in 
      transformed cells, however, showed patterns different from those in 
      nontransformed cells. Southern blot analyses indicated no detectable alterations 
      in myc, ras, Rb, or p53 genes. Further studies of chromosome 17 by using in situ 
      hybridization with unique sequence p53 gene probe and a centromere probe showed 
      no loss of p53 gene in transformed cells. Experimental results from cell fusion 
      studies indicated that the transforming gene(s) is recessive. The role of genomic 
      instability and tumor suppressor gene(s) in radiogenic transformation of human 
      breast cells remains to be identified.
FAU - Yang, T C
AU  - Yang TC
AD  - Radiation Biophysics Laboratory, NASA Johnson Space Center, Houston, Texas, USA.
FAU - Georgy, K A
AU  - Georgy KA
FAU - Tavakoli, A
AU  - Tavakoli A
FAU - Craise, L M
AU  - Craise LM
FAU - Durante, M
AU  - Durante M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Oncol Investig
JT  - Radiation oncology investigations
JID - 9437448
RN  - 9007-49-2 (DNA)
MH  - Breast/pathology/*radiation effects
MH  - Cell Fusion
MH  - Cell Line, Transformed
MH  - *Cell Transformation, Neoplastic
MH  - Chromosome Aberrations
MH  - Chromosomes/genetics/radiation effects
MH  - *Cosmic Radiation
MH  - DNA/genetics/radiation effects
MH  - Epithelium/pathology/radiation effects
MH  - Female
MH  - Genes, Recessive/genetics
MH  - Genes, Tumor Suppressor/genetics
MH  - Heavy Ions
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Linear Energy Transfer
MH  - Particle Accelerators
OID - NASA: 00023048
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- NASA JSC
EDAT- 1996/01/01 00:00
MHDA- 2001/09/11 10:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2001/09/11 10:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/roi.2970030631 [doi]
PST - ppublish
SO  - Radiat Oncol Investig. 1996;3:412-9. doi: 10.1002/roi.2970030631.