PMID- 11543885
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190901
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 98
IP  - 1
DP  - 2001 Oct
TI  - Quantitative TaqMan RT-PCR for the detection and differentiation of European and 
      North American strains of porcine reproductive and respiratory syndrome virus.
PG  - 63-75
AB  - Since two different types of porcine reproductive and respiratory syndrome virus 
      (PRRSV), the European (EU) and the North American (US) strain, occur or coexist 
      in European swine herds, their rapid and reliable detection and differentiation 
      is essential for disease surveillance. A quantitative TaqMan reverse 
      transcription-polymerase chain reaction (RT-PCR) is described for PRRSV detection 
      and strain differentiation. Sensitivity and specificity were compared with a 
      conventional PRRSV RT-PCR and to the detection of both PRRSV types in cell 
      cultures and both were found to be equal or superior to the reference methods. 
      Reproducibility was tested and proved that the assay was very reliable. Standard 
      dilutions included in each test allowed absolute quantitation of the amount of 
      viral RNA. The TaqMan assay described below is time-saving, easy to handle, 
      exhibits a decreased risk of cross-contamination and is highly sensitive and 
      specific. It is, therefore, considered to be a powerful tool for the rapid 
      detection and differentiation of PRRSV.
FAU - Egli, C
AU  - Egli C
AD  - Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH-3147, 
      Mittelhausern, Switzerland.
FAU - Thur, B
AU  - Thur B
FAU - Liu, L
AU  - Liu L
FAU - Hofmann, M A
AU  - Hofmann MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.- (Taq Polymerase)
SB  - IM
MH  - Animals
MH  - Europe
MH  - Fluorescent Dyes
MH  - North America
MH  - Polymerase Chain Reaction/methods
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/genetics/*isolation & purification
MH  - RNA, Viral/analysis
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine/*virology
MH  - Taq Polymerase
EDAT- 2001/09/07 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/07 10:00
PHST- 2001/09/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/07 10:00 [entrez]
AID - S0166093401003585 [pii]
AID - 10.1016/s0166-0934(01)00358-5 [doi]
PST - ppublish
SO  - J Virol Methods. 2001 Oct;98(1):63-75. doi: 10.1016/s0166-0934(01)00358-5.