PMID- 11544144
OWN - NLM
STAT- MEDLINE
DCOM- 20011217
LR  - 20191105
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 130
IP  - 1
DP  - 2001 Sep
TI  - Catalytic and immunochemical properties of hepatic cytochrome P450 1A in three 
      avian species treated with beta-naphthoflavone or isosafrole.
PG  - 67-83
AB  - Induction of cytochrome P450 1A (CYP1A) can be used as a biomarker of exposure to 
      planar halogenated aromatic hydrocarbons (PHAHs). Our objective was to 
      characterize the induction of CYP1A activity and protein in three avian species 
      following in vivo treatment with beta-naphthoflavone (BNF) and/or isosafrole. 
      Alkoxyresorufin-O-dealkylase (alk-ROD) activities of hepatic microsomes from 
      Herring Gulls (Larus argentatus) (HGs), Double-crested Cormorants (Phalacrocorax 
      auritus) (DCCs) and chickens (Gallus domesticus) were measured using ethoxy-, 
      methoxy-, pentoxy- and benzyloxy-resorufin, in the presence and absence of the 
      inhibitors ellipticine or furafylline. Immunoreactivity of microsomal proteins 
      with antibodies to several CYP1A proteins was investigated. CYP1A protein and 
      alk-ROD activities of HGs and DCCs, but not chickens, were induced by isosafrole. 
      Ellipticine was a potent and non-selective inhibitor of alk-ROD activity in all 
      three species, while furafylline inhibition of alk-ROD activities varied among 
      species and treatments. In all three species, BNF induced a protein 
      immunoreactive with monoclonal antibody to CYP1A1 from the marine fish Stenotomus 
      chrysops (scup), but a CYP1A2-like protein was not detected in avian microsomes 
      probed with polyclonal antibodies to mouse CYP1A2. Variations in responses among 
      avian species indicate that CYP1A proteins and substrate specificities should be 
      characterized for each species used in PHAH biomonitoring programs.
FAU - Verbrugge, L A
AU  - Verbrugge LA
AD  - Department of Fisheries and Wildlife, Pesticide Research Center and Institute for 
      Environmental Toxicology, Michigan State University, East Lansing, MI 48824-1222, 
      USA.
FAU - Giesy, J P
AU  - Giesy JP
FAU - Verbrugge, D A
AU  - Verbrugge DA
FAU - Woodin, B R
AU  - Woodin BR
FAU - Stegeman, J J
AU  - Stegeman JJ
LA  - eng
GR  - ES-04911/ES/NIEHS NIH HHS/United States
GR  - P42-ES07381/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Antibodies)
RN  - 0 (Cytochrome P-450 CYP1A2 Inhibitors)
RN  - 0 (Ellipticines)
RN  - 0 (Enzyme Inhibitors)
RN  - 117VLW7484 (ellipticine)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
RN  - RSB34337V9 (Safrole)
RN  - W6337429LF (isosafrole)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Catalysis
MH  - Chickens
MH  - Cytochrome P-450 CYP1A1/*biosynthesis
MH  - Cytochrome P-450 CYP1A2/immunology/*metabolism
MH  - Cytochrome P-450 CYP1A2 Inhibitors
MH  - Ellipticines/pharmacology
MH  - Enzyme Induction
MH  - Enzyme Inhibitors/*pharmacology
MH  - Hepatocytes/*drug effects/enzymology
MH  - Immunoblotting
MH  - Immunochemistry
MH  - Safrole/*pharmacology
MH  - Species Specificity
MH  - beta-Naphthoflavone/*pharmacology
EDAT- 2001/09/07 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/07 10:00
PHST- 2001/09/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/07 10:00 [entrez]
AID - S1532-0456(01)00221-6 [pii]
AID - 10.1016/s1532-0456(01)00221-6 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2001 Sep;130(1):67-83. doi: 
      10.1016/s1532-0456(01)00221-6.