PMID- 11544262
OWN - NLM
STAT- MEDLINE
DCOM- 20020110
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 48
DP  - 2001 Nov 30
TI  - The human low affinity Fcgamma receptors IIa, IIb, and III bind IgG with fast 
      kinetics and distinct thermodynamic properties.
PG  - 44898-904
AB  - Fcgamma receptors (FcgammaRs) are expressed on all immunologically active cells. 
      They bind the Fc portion of IgG, thereby triggering a range of immunological 
      functions. We have used surface plasmon resonance to analyze the kinetic and 
      thermodynamic properties of the interactions between the ectodomains of human low 
      affinity FcgammaRs (FcgammaRIIa, FcgammaRIIb, and FcgammaRIIIb-NA2) and IgG1 or 
      the Fc fragment of IgG1. All three receptors bind Fc or IgG with similarly low 
      affinities (K(D) approximately 0.6-2.5 microm) and fast kinetics, suggesting that 
      FcgammaR-mediated recognition of aggregated IgG and IgG-coated particles or cells 
      is mechanistically similar to cell-cell recognition. Interestingly, the Fc 
      receptors exhibit distinct thermodynamic properties. Whereas the binding of the 
      FcgammaRIIa and FcgammaRIIb to Fc is driven by favorable entropic and enthalpic 
      changes, the binding of FcgammaRIII is characterized by highly unfavorable 
      entropic changes. Although the structural bases for these differences remain to 
      be determined, they suggest that the molecular events coupled to the binding 
      differ among the low affinity FcgammaRs.
FAU - Maenaka, K
AU  - Maenaka K
AD  - Structural Biology Center, National Institute of Genetics, Mishima, Shizuoka 
      411-8540, Japan. kmaenaka@lab.nig.ac.jp
FAU - van der Merwe, P A
AU  - van der Merwe PA
FAU - Stuart, D I
AU  - Stuart DI
FAU - Jones, E Y
AU  - Jones EY
FAU - Sondermann, P
AU  - Sondermann P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010905
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (FCGR3B protein, human)
RN  - 0 (Fc gamma receptor IIA)
RN  - 0 (Fc gamma receptor IIB)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antigens, CD/chemistry/*metabolism
MH  - Entropy
MH  - GPI-Linked Proteins
MH  - Humans
MH  - Immunoglobulin G/metabolism
MH  - Kinetics
MH  - Models, Molecular
MH  - Protein Binding
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Receptors, IgG/chemistry/*metabolism
MH  - Surface Plasmon Resonance
MH  - Temperature
MH  - Thermodynamics
MH  - Time Factors
EDAT- 2001/09/07 10:00
MHDA- 2002/01/11 10:01
CRDT- 2001/09/07 10:00
PHST- 2001/09/07 10:00 [pubmed]
PHST- 2002/01/11 10:01 [medline]
PHST- 2001/09/07 10:00 [entrez]
AID - S0021-9258(19)82693-5 [pii]
AID - 10.1074/jbc.M106819200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Nov 30;276(48):44898-904. doi: 10.1074/jbc.M106819200. Epub 
      2001 Sep 5.