PMID- 11544442
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 72
IP  - 4
DP  - 2001 Aug 27
TI  - Promoter-region alleles of the TNF-alpha and IL-10 genes have no effect on 
      pretransplant alloantibody production.
PG  - 739-42
AB  - BACKGROUND: The presence of high levels of alloantibodies are known to be a risk 
      factor in renal graft outcome. Expression level polymorphisms in cytokine genes 
      are also thought to have an effect on allograft outcome, but the studies 
      examining this have been inconsistent. This may be due to center-specific 
      differences in immunosuppressive protocols. Therefore, we studied the effects of 
      these polymorphisms on pretransplant class I alloantibody production in 
      nonexogenously immunosuppressed candidates. METHODS: Tumor necrosis factor-alpha 
      (TNF-alpha) and interleukin-10 (IL-10) gene polymorphisms were assayed 
      genotypically by PCR-SSP on 177 renal transplant candidates. Candidates with a 
      peak goat antihuman immunoglobulin-enhanced T-cell panel reactive antibody (PRA) 
      of >or=10% were considered to be positive for alloantibody (32% of 177 total). 
      RESULTS: Previous transplants, transfusions, or pregnancies were all associated 
      with alloantibody production, but TNF-alpha and IL-10 phenotypes were not. High 
      levels of alloantibody production (peak PRA >50%) were also not effected by 
      cytokine phenotype. CONCLUSIONS: These data suggest that differences in TNF-alpha 
      and IL-10 phenotype do not effect a patient's likelihood of becoming sensitized 
      by transfusions, pregnancies, and prior transplants.
FAU - Hahn, A B
AU  - Hahn AB
AD  - Department of Surgery, Albany Medical College, New York 12208, USA.
FAU - Kasten-Jolly, J C
AU  - Kasten-Jolly JC
FAU - Constantino, D M
AU  - Constantino DM
FAU - Graffunder, E
AU  - Graffunder E
FAU - Conti, D J
AU  - Conti DJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Isoantibodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - *Alleles
MH  - Female
MH  - Humans
MH  - Interleukin-10/*genetics
MH  - Isoantibodies/*biosynthesis
MH  - *Kidney Transplantation
MH  - Male
MH  - Phenotype
MH  - Polymorphism, Genetic
MH  - Promoter Regions, Genetic/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2001/09/07 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/09/07 10:00
PHST- 2001/09/07 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/09/07 10:00 [entrez]
AID - 10.1097/00007890-200108270-00032 [doi]
PST - ppublish
SO  - Transplantation. 2001 Aug 27;72(4):739-42. doi: 10.1097/00007890-200108270-00032.