PMID- 11545397
OWN - NLM
STAT- MEDLINE
DCOM- 20020128
LR  - 20131121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 22
IP  - 13
DP  - 2001 Aug
TI  - Head-column field-amplified sample stacking in capillary electrophoresis for the 
      determination of cimetidine, famotidine, nizatidine, and ranitidine-HCl in 
      plasma.
PG  - 2717-22
AB  - In this study, low concentrations of histamine2-receptor (H2-)antagonists were 
      effected across a water plug, with separation taking place in a binary buffer 
      comprising ethylene glycol and NaH2PO4 (pH 5.0), and detection at 214 nm. 
      Liquid-liquid extraction with ethyl acetate- isopropanol is shown to provide 
      extracts that are sufficiently clean. The calibration curves were linear over a 
      concentration range of 0.1-2.00 microg/mL cimetidine, 0.2-5.0 microg/mL 
      ranitidine-HCl, 0.3-5.0 microg/mL nizatidine, and 0.1-3.0 microg/mL famotidine. 
      Mean recoveries were > 82%, while the intra- and interday relative standard 
      deviations (RSDs) and relative errors (REs) were all < 13%. The method is 
      sensitive with a detection limit of 3 ng/mL cimetidine, 30 ng/mL ranitidine HCl, 
      50 ng/mL nizatidine and 10 ng/mL famotidine (S/N = 3, electric-driven injection 
      90 s). This newly developed capillary electrophoresis (CE) method was applied for 
      the determination of analytes extracted from plasma taken from a volunteer dosing 
      a cimetidine, ranitidine, and nizatidine tablet simultaneously. These three 
      H2-antagonists can be detected in real samples by this method, excluding the low 
      dosing of famotidine tablet.
FAU - Wu, S M
AU  - Wu SM
AD  - School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Taiwan. 
      shmewu@kmu.edu.tw
FAU - Ho, Y H
AU  - Ho YH
FAU - Wu, H L
AU  - Wu HL
FAU - Chen, S H
AU  - Chen SH
FAU - Ko, H S
AU  - Ko HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Buffers)
RN  - 0 (Histamine H2 Antagonists)
RN  - 0 (Phosphates)
RN  - 059QF0KO0R (Water)
RN  - 5QZO15J2Z8 (Famotidine)
RN  - 80061L1WGD (Cimetidine)
RN  - 884KT10YB7 (Ranitidine)
RN  - FC72KVT52F (Ethylene Glycol)
RN  - P41PML4GHR (Nizatidine)
SB  - IM
MH  - Buffers
MH  - Cimetidine/*blood
MH  - Electric Conductivity
MH  - Electrophoresis, Capillary/*methods/standards
MH  - Ethylene Glycol
MH  - Famotidine/*blood
MH  - Histamine H2 Antagonists/*blood
MH  - Humans
MH  - Nizatidine/*blood
MH  - Phosphates
MH  - Ranitidine/*blood
MH  - Sensitivity and Specificity
MH  - Time Factors
MH  - Water
EDAT- 2001/09/08 10:00
MHDA- 2002/01/29 10:01
CRDT- 2001/09/08 10:00
PHST- 2001/09/08 10:00 [pubmed]
PHST- 2002/01/29 10:01 [medline]
PHST- 2001/09/08 10:00 [entrez]
AID - 10.1002/1522-2683(200108)22:13<2717::AID-ELPS2717>3.0.CO;2-2 [pii]
AID - 10.1002/1522-2683(200108)22:13<2717::AID-ELPS2717>3.0.CO;2-2 [doi]
PST - ppublish
SO  - Electrophoresis. 2001 Aug;22(13):2717-22. doi: 
      10.1002/1522-2683(200108)22:13<2717::AID-ELPS2717>3.0.CO;2-2.