PMID- 11545412
OWN - NLM
STAT- MEDLINE
DCOM- 20020128
LR  - 20041117
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 22
IP  - 13
DP  - 2001 Aug
TI  - Proteome analysis of a human heptocellular carcinoma cell line, HCC-M: an update.
PG  - 2804-11
AB  - Recently, we reported the proteome analysis of a human hepatocellular carcinoma 
      cell line, HCC-M (Electrophoresis 2000, 21, 1787-1813), using two-dimensional gel 
      electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of 
      flight-mass spectrometry (MALDI-TOF-MS). From a total of 408 unique spots excised 
      from the 2-DE gel, 301 spots yielded good MALDI spectra. Out of these, 272 spots 
      had matches returned from the database search leading to the identification of 
      these proteins. Here, we report the results on the identification of the 
      remaining 29 spots using nanoelectrospray ionization-tandem mass spectrometry 
      (nESI-MS/MS). First, "peptide tag sequencing" was performed to obtain partial 
      amino acid sequences of the peptides to search the SWISS-PROTand NCBI 
      nonredundant protein databases. Spots that were still not able to find any 
      matches from the databases were subjected to de novo peptide sequencing. The 
      tryptic peptide sequences were used to search for homologues in the protein and 
      nucleotide databases with the NCBI Basic Local Alignment Search Tool (BLAST), 
      which was essential for the characterization of novel or post-translationally 
      modified proteins. Using this approach, all the 29 spots were unambiguously 
      identified. Among them, phosphotyrosyl phosphatase activator (PTPA), RNA-binding 
      protein regulatory subunit, replication protein A 32 kDa subunit (RP-A) and 
      N-acetylneuraminic acid phosphate synthase were reported to be cancer-related 
      proteins.
FAU - Ou, K
AU  - Ou K
AD  - Bioprocessing Technology Center, National University of Singapore. 
      btcoukl@nus.edu.sg
FAU - Seow, T K
AU  - Seow TK
FAU - Liang, R C
AU  - Liang RC
FAU - Ong, S E
AU  - Ong SE
FAU - Chung, M C
AU  - Chung MC
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Carcinoma, Hepatocellular/*chemistry
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Liver Neoplasms/*chemistry
MH  - Neoplasm Proteins/*analysis
MH  - Proteome/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Tumor Cells, Cultured
EDAT- 2001/09/08 10:00
MHDA- 2002/01/29 10:01
CRDT- 2001/09/08 10:00
PHST- 2001/09/08 10:00 [pubmed]
PHST- 2002/01/29 10:01 [medline]
PHST- 2001/09/08 10:00 [entrez]
AID - 10.1002/1522-2683(200108)22:13<2804::AID-ELPS2804>3.0.CO;2-G [pii]
AID - 10.1002/1522-2683(200108)22:13<2804::AID-ELPS2804>3.0.CO;2-G [doi]
PST - ppublish
SO  - Electrophoresis. 2001 Aug;22(13):2804-11. doi: 
      10.1002/1522-2683(200108)22:13<2804::AID-ELPS2804>3.0.CO;2-G.