PMID- 11549056
OWN - NLM
STAT- MEDLINE
DCOM- 20020409
LR  - 20131121
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 10
IP  - 4-5
DP  - 2001
TI  - Insertion of a suicide gene into an immortalized human hepatocyte cell line.
PG  - 373-6
AB  - For developing a bioartificial liver (BAL) device, an attractive alternative to 
      the primary human hepatocytes would be the use of highly differentiated 
      immortalized human hepatocytes with a safeguard. To test the feasibility, the 
      primary human hepatocytes were immortalized by a plasmid SV3neo encoding simian 
      virus 40 large T antigen (SV40Tag) gene. A highly differentiated hepatocyte line 
      OUMS-29 was established. A suicide gene of herpes simplex virus-thymidine kinase 
      (HSV-TK) was retrovirally introduced into OUMS-29 cells as a safeguard for 
      clinical application. One of the resulting HSV-TK-positive cell lines, 
      OUMS-29/tk, grew in chemically defined serum-free medium with the gene expression 
      of differentiated liver functions. OUMS-29/tk cells were 100 times more sensitive 
      to ganciclovir compared with unmodified OUMS-29 cells in in vitro experiments. We 
      have established a tightly regulated immortalized human hepatocyte cell line. 
      Essentially unlimited availability of OUMS-29/tk cells may be clinically useful 
      for BAL therapy.
FAU - Kobayashi, N
AU  - Kobayashi N
AD  - First Department of Surgery, Okayama University Medical School, Japan. 
      immortal@md.okayama-u.ac.jp
FAU - Noguchi, H
AU  - Noguchi H
FAU - Totsugawa, T
AU  - Totsugawa T
FAU - Watanabe, T
AU  - Watanabe T
FAU - Matsumura, T
AU  - Matsumura T
FAU - Fujiwara, T
AU  - Fujiwara T
FAU - Miyazaki, M
AU  - Miyazaki M
FAU - Fukaya, K
AU  - Fukaya K
FAU - Namba, M
AU  - Namba M
FAU - Tanaka, N
AU  - Tanaka N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Antiviral Agents)
RN  - 0 (Culture Media, Serum-Free)
RN  - EC 2.7.1.21 (Thymidine Kinase)
RN  - P9G3CKZ4P5 (Ganciclovir)
SB  - IM
MH  - Antiviral Agents/pharmacology
MH  - *Cell Line
MH  - Cell Size
MH  - Culture Media, Serum-Free
MH  - Fetus
MH  - Ganciclovir/pharmacology
MH  - Gene Expression/physiology
MH  - *Gene Transfer Techniques
MH  - Hepatocytes/cytology/drug effects/*physiology
MH  - Humans
MH  - Liver
MH  - *Liver, Artificial
MH  - Male
MH  - Simplexvirus/*enzymology/genetics
MH  - Thymidine Kinase/*genetics
EDAT- 2001/09/11 10:00
MHDA- 2002/04/18 10:01
CRDT- 2001/09/11 10:00
PHST- 2001/09/11 10:00 [pubmed]
PHST- 2002/04/18 10:01 [medline]
PHST- 2001/09/11 10:00 [entrez]
PST - ppublish
SO  - Cell Transplant. 2001;10(4-5):373-6.