PMID- 11549113
OWN - NLM
STAT- MEDLINE
DCOM- 20020214
LR  - 20191210
IS  - 0893-5785 (Print)
IS  - 0893-5785 (Linking)
VI  - 14
IP  - 4
DP  - 2001 Aug
TI  - Production of melanocyte-specific antibodies to human melanosomal proteins: 
      expression patterns in normal human skin and in cutaneous pigmented lesions.
PG  - 289-97
AB  - Multiple factors affect skin pigmentation, including those that regulate 
      melanocyte and/or keratinocyte function. Such factors, particularly those that 
      operate at the level of the melanosome, are relatively well characterized in 
      mice, but the expression and function of structural and enzymatic proteins in 
      melanocytes in human skin are not as well known. Some years ago, we generated 
      peptide-specific antibodies to murine melanosomal proteins that proved to be 
      instrumental in elucidating melanocyte development and differentiation in mice, 
      but cross-reactivity of those antibodies with the corresponding human proteins 
      often was weak or absent. In an effort to characterize the roles of melanosomal 
      proteins in human skin pigmentation, and to understand the underlying 
      mechanism(s) of abnormal skin pigmentation, we have now generated polyclonal 
      antibodies against the human melanocyte-specific markers, tyrosinase, 
      tyrosinase-related protein (TYRP1), Dopachrome tautomerase (DCT) and Pmel17 
      (SILV, also known as GP100). We used these antibodies to determine the 
      distribution and function of melanosomal proteins in normal human skin (adult and 
      newborn) and in various cutaneous pigmented lesions, such as intradermal nevi, 
      lentigo simplex, solar lentigines and malignant melanomas. We also examined 
      cytokeratin expression in these same samples to assess keratinocyte distribution 
      and function. Immunohistochemical staining reveals distinct patterns of 
      melanocyte distribution and function in normal skin and in various types of 
      cutaneous pigmented lesions. Those differences in the expression patterns of 
      melanocyte markers provide important clues to the roles of melanocytes in normal 
      and in disrupted skin pigmentation.
FAU - Virador, V
AU  - Virador V
AD  - Laboratory of Cell Biology, National Cancer Institute, National Institutes of 
      Health, Bethesda, Maryland 20892, USA.
FAU - Matsunaga, N
AU  - Matsunaga N
FAU - Matsunaga, J
AU  - Matsunaga J
FAU - Valencia, J
AU  - Valencia J
FAU - Oldham, R J
AU  - Oldham RJ
FAU - Kameyama, K
AU  - Kameyama K
FAU - Peck, G L
AU  - Peck GL
FAU - Ferrans, V J
AU  - Ferrans VJ
FAU - Vieira, W D
AU  - Vieira WD
FAU - Abdel-Malek, Z A
AU  - Abdel-Malek ZA
FAU - Hearing, V J
AU  - Hearing VJ
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Pigment Cell Res
JT  - Pigment cell research
JID - 8800247
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (PMEL protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Pmel protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (gp100 Melanoma Antigen)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (TYRP1 protein, human)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antibody Specificity
MH  - Cells, Cultured
MH  - Frozen Sections
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant, Newborn
MH  - Intramolecular Oxidoreductases/analysis/immunology
MH  - Keratinocytes/chemistry/enzymology/immunology
MH  - Lentigo/*pathology
MH  - Melanocytes/*chemistry/enzymology/*immunology
MH  - Melanoma/pathology
MH  - Melanosomes/chemistry/enzymology/immunology
MH  - *Membrane Glycoproteins
MH  - Molecular Sequence Data
MH  - Monophenol Monooxygenase/analysis/immunology
MH  - Nevus, Intradermal/pathology
MH  - *Oxidoreductases
MH  - Peptide Fragments/chemistry/immunology
MH  - Proteins/analysis/immunology
MH  - Rabbits
MH  - Skin/*chemistry/cytology/enzymology
MH  - Skin Neoplasms/pathology
MH  - Skin Pigmentation
MH  - gp100 Melanoma Antigen
EDAT- 2001/09/11 10:00
MHDA- 2002/02/15 10:01
CRDT- 2001/09/11 10:00
PHST- 2001/09/11 10:00 [pubmed]
PHST- 2002/02/15 10:01 [medline]
PHST- 2001/09/11 10:00 [entrez]
AID - 10.1034/j.1600-0749.2001.140410.x [doi]
PST - ppublish
SO  - Pigment Cell Res. 2001 Aug;14(4):289-97. doi: 10.1034/j.1600-0749.2001.140410.x.