PMID- 11550279
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 2
DP  - 2001 Oct
TI  - Loss of chromosome arms 3p and 9p and inactivation of P16 (INK4a) in normal 
      epithelium of patients with primary lung cancer.
PG  - 119-25
AB  - The accumulation of genetic alterations in the respiratory epithelium may give 
      rise to cancer and often is accompanied by a series of histologic alterations 
      over a period of several years. Recent studies have identified some molecular 
      alterations in histologically normal-appearing epithelium among patients with 
      lung cancer. To extend these observations, we investigated clonal genetic 
      alterations by using fluorescence in situ hybridization (FISH) analysis and 
      immunohistochemistry in 69 biopsy samples of histologically normal-appearing 
      bronchial epithelium from 22 patients with or without lung cancer. Thirty-seven 
      biopsy specimens from 13 patients were examined for loss of 3p14, and 48 biopsy 
      specimens from 18 patients were examined for loss at 9p21 by FISH. P16(INK4a) 
      expression was analyzed in 54 biopsy samples from 19 patients. In at least one 
      biopsy specimen from five of the 13 patients with primary lung cancer, FISH or 
      immunohistochemistry detected loss of the 3p14 or 9p21 region. In contrast, no 
      alterations were detected for the same regions in the nine patients without 
      primary lung cancer. Our results support the concept that the normal epithelial 
      surface of large bronchi of patients with lung cancer has molecular changes 
      suggestive of the outgrowth of numerous clonal foci.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Caballero, O L
AU  - Caballero OL
AD  - Department of Otolaryngology-Head and Neck Surgery, Division of Head and Neck 
      Cancer Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
FAU - Cohen, D
AU  - Cohen D
FAU - Liu, Q
AU  - Liu Q
FAU - Esteller, M
AU  - Esteller M
FAU - Bonacum, J
AU  - Bonacum J
FAU - White, P
AU  - White P
FAU - Engles, J
AU  - Engles J
FAU - Yochem, R
AU  - Yochem R
FAU - Herman, J G
AU  - Herman JG
FAU - Westra, W H
AU  - Westra WH
FAU - Lengauer, C
AU  - Lengauer C
FAU - Sidransky, D
AU  - Sidransky D
FAU - Jen, J
AU  - Jen J
LA  - eng
GR  - CA 58184/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
SB  - IM
MH  - Bronchi/cytology/metabolism
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics/metabolism
MH  - Female
MH  - *Gene Silencing
MH  - *Genes, p16
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Respiratory Mucosa/cytology/*metabolism
EDAT- 2001/09/11 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/11 10:00
PHST- 2001/09/11 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/11 10:00 [entrez]
AID - 10.1002/gcc.1173 [pii]
AID - 10.1002/gcc.1173 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Oct;32(2):119-25. doi: 10.1002/gcc.1173.