PMID- 11550287
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 2
DP  - 2001 Oct
TI  - Establishment of a cell line with AML1-MTG8, TP53, and TP73 abnormalities from 
      acute myelogenous leukemia.
PG  - 182-7
AB  - Gene alterations accumulate during the progression of acute myelogenous leukemia 
      (AML) to a malignant clone. Here, a new myeloid cell line, designated YSK-21, 
      with the balanced t(8;21)(q22;q22) and the unbalanced der(1)t(1;17)(p36;q21), was 
      established. YSK-21 grows well in a medium containing recombinant human 
      granulocyte colony-stimulating factor (rhG-CSF), granulocyte-macrophage 
      colony-stimulating factor (rhGM-CSF), or interleukin-3 (rhIL-3). Molecular 
      analysis using the reverse transcriptase-polymerase chain reaction (RT-PCR) and 
      fluorescence in situ hybridization (FISH) revealed that t(8;21)(q22;q22) resulted 
      in an AML1-MTG8 fusion transcript. FISH and spectral karyotyping (SKY) in 
      conjunction with G-banding analysis revealed a der(1)t(1;17)(p36;q21) chromosomal 
      translocation, which appeared in the clone developed from the original leukemic 
      cells. Molecular analysis of the TP73 gene on 1p36 and the TP53 gene revealed a 
      deletion of one-allele in TP73 with partial demethylation of another allele in 
      the initial clone of YSK, and a point mutation consisting of an A-->T 
      substitution in codon 288 of the TP53 gene in the developed clone of YSK-21. 
      YSK-21 cells, expressing aberrant AML1-MTG8, TP53, and TP73 protein molecules, 
      may be useful for elucidating the pathophysiology of these aberrant proteins and 
      for studying the der(1)t(1;17)(p36;q21) chromosomal translocation.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Inokuchi, K
AU  - Inokuchi K
AD  - Division of Hematology, Department of Internal Medicine, Nippon Medical School, 
      1-1-5 Sendagi, Bunkyo-ku, Tokyo 113, Japan. inokuchi@nms.ac.jp
FAU - Hamaguchi, H
AU  - Hamaguchi H
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Yamaguchi, H
AU  - Yamaguchi H
FAU - Tanosaki, S
AU  - Tanosaki S
FAU - Dan, K
AU  - Dan K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (AML1-ETO fusion protein, human)
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RUNX1 Translocation Partner 1 Protein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Culture Techniques/methods
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Mutation/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - RUNX1 Translocation Partner 1 Protein
MH  - Transcription Factors/*genetics
MH  - Tumor Cells, Cultured/chemistry/metabolism/*pathology
EDAT- 2001/09/11 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/11 10:00
PHST- 2001/09/11 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/11 10:00 [entrez]
AID - 10.1002/gcc.1181 [pii]
AID - 10.1002/gcc.1181 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Oct;32(2):182-7. doi: 10.1002/gcc.1181.