PMID- 11550806
OWN - NLM
STAT- MEDLINE
DCOM- 20020225
LR  - 20190906
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 293
IP  - 7
DP  - 2001 Jul
TI  - Expression of IL-18 in psoriasis.
PG  - 334-42
AB  - Interleukin-18 (IL-18) is a novel cytokine that plays an important role in the 
      T-helper 1 (Th1) response, primarily via its ability to induce IFN-gamma 
      production in T cells and NK cells. Human keratinocytes produce IL-18, as do 
      monocytes and macrophages, which are the two major sources of this molecule. It 
      is thought that IL-18 derived from keratinocytes might be involved in the 
      cutaneous Th1-type immune response. In the present study, we investigated the 
      expression of IL-18 in psoriatic lesional skin and attempted to determine whether 
      immunoreactive IL-18 in crude extracts of psoriatic scales is processed to the 
      mature, active form. Immunohistochemical and RT-PCR analysis showed that the 
      expression of IL-18 was increased in psoriatic lesional skin relative to that in 
      normal skin. Western blotting and an ELISA for IL-18 in combination demonstrated 
      that the immunoreactive IL-18 in extracts of psoriatic scales contained the 
      mature form of IL-18, but most of the IL-18 was pro-IL-18. No bioactivity of 
      IL-18 or IFN-gamma inducibility in human PBMC could be detected in psoriatic 
      scales. Taken together, these findings indicate that keratinocyte-derived IL-18 
      participates in the development of the Th1 response in psoriatic lesions, and 
      that its bioactivity appears to be tightly regulated in cutaneous inflammation.
FAU - Ohta, Y
AU  - Ohta Y
AD  - Department of Dermatology, Kitasato University School of Medicine, 
      Sagamihara-shi, Kanagawa, Japan. yukiohta@med.kitasato-u.ac.jp
FAU - Hamada, Y
AU  - Hamada Y
FAU - Katsuoka, K
AU  - Katsuoka K
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (interleukin-18 binding protein)
SB  - IM
MH  - Biological Assay
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins
MH  - Interleukin-18/*metabolism
MH  - Psoriasis/*metabolism
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/09/12 10:00
MHDA- 2002/02/28 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
AID - 10.1007/s004030100240 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 2001 Jul;293(7):334-42. doi: 10.1007/s004030100240.