PMID- 11550906
OWN - NLM
STAT- MEDLINE
DCOM- 20020314
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 44
IP  - 4
DP  - 2001 Aug
TI  - Genomic interspersions determine the size and complexity of transgene loci in 
      transgenic plants produced by microprojectile bombardment.
PG  - 691-7
AB  - The structure of transgene loci in six transgenic allohexaploid oat (Avena sativa 
      L.) lines produced using microprojectile bombardment was characterized using 
      fluorescence in situ hybridization (FISH) on extended DNA fibers (fiber-FISH). 
      The transgene loci in five lines were composed of multiple copies of delivered 
      DNA interspersed with genomic DNA fragments ranging in size from ca. 3 kb to at 
      least several hundred kilobases, and in greater numbers than detected using 
      Southern blot analysis. Although Southern analysis predicted that the transgene 
      locus in one line consisted of long tandem repeats of the delivered DNA, 
      fiber-FISH revealed that the locus actually contained multiple genomic 
      interspersions. These observations indicated that transgene locus size and 
      structure were determined by the number of transgene copies and, possibly to a 
      greater extent, the number and the length of interspersing genomic DNA sequences 
      within the locus. Large genomic interspersions detected in several lines were 
      most likely the products of chromosomal breakage induced either by tissue culture 
      conditions or, more likely, by DNA delivery into the nucleus using 
      microprojectile bombardment. We propose that copies of transgene along with other 
      extrachromosomal DNA fragments are used as patches to repair double-strand breaks 
      (DSBs) in the plant genome resulting in the formation of transgene loci.
FAU - Svitashev, S K
AU  - Svitashev SK
AD  - Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, 
      University of Minnesota, St Paul 55108, USA.
FAU - Somers, D A
AU  - Somers DA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Avena/genetics
MH  - Blotting, Southern
MH  - DNA/metabolism
MH  - Genotype
MH  - In Situ Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - *Plants, Genetically Modified
MH  - Plasmids/metabolism
MH  - *Transgenes
EDAT- 2001/09/12 10:00
MHDA- 2002/03/15 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/03/15 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
PST - ppublish
SO  - Genome. 2001 Aug;44(4):691-7.