PMID- 11550910
OWN - NLM
STAT- MEDLINE
DCOM- 20020314
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 44
IP  - 4
DP  - 2001 Aug
TI  - The genetic identity of alien chromosomes in potato breeding lines revealed by 
      sequential GISH and FISH analyses using chromosome-specific cytogenetic DNA 
      markers.
PG  - 729-34
AB  - Genomic in situ hybridization (GISH) is one of the most popular and effective 
      techniques for detecting alien chromatin introgressed into breeding lines; 
      however, GISH analysis alone does not reveal the genetic identity of the alien 
      chromosomes. We previously isolated a set of bacterial artificial chromosomes 
      (BACs) specific to each of the 12 potato chromosomes. These BAC clones can be 
      used as chromosome-specific cytogenetic DNA markers (CSCDMs) for potato 
      chromosome identification. Here we demonstrate that GISH and fluorescence in situ 
      hybridization (FISH), using CSCDMs, can be performed sequentially on the same 
      chromosome preparations. Somatic metaphase chromosomes prepared using an 
      enzymatic digestion and "flame-drying" procedure allows repeated probing up to 
      five times without significant damage to chromosome morphology. The sequential 
      GISH and FISH analyses reveal the genomic origin and genetic identity of the 
      alien chromosomes in a single experiment and also determine whether an alien 
      chromosome has been added to the genetic background of potato or is substituting 
      for a homoeologous potato chromosome. The sequential GISH and FISH procedures 
      should be widely applicable for germplasm characterization, especially in plant 
      species with small-sized chromosomes.
FAU - Dong, F
AU  - Dong F
AD  - Department of Horticulture, University of Wisconsin-Madison, 53706, USA.
FAU - McGrath, J M
AU  - McGrath JM
FAU - Helgeson, J P
AU  - Helgeson JP
FAU - Jiang, J
AU  - Jiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosomes/ultrastructure
MH  - DNA/*metabolism
MH  - *Genetic Markers
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Solanum tuberosum/*genetics
EDAT- 2001/09/12 10:00
MHDA- 2002/03/15 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/03/15 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
PST - ppublish
SO  - Genome. 2001 Aug;44(4):729-34.