PMID- 11551490
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 496
IP  - 1-2
DP  - 2001 Sep 20
TI  - Effect of chlorophyllin on chromium trioxide-induced micronuclei in polychromatic 
      erythrocytes in mouse peripheral blood.
PG  - 145-51
AB  - The effect of chlorophyllin on micronucleated polychromatic erythrocytes (MN-PCE) 
      induction by chromium trioxide (CrO(3)) exposure in peripheral blood of mice was 
      studied. Animals were treated with a single intraperitoneal dose of chlorophyllin 
      (CHL) (20mg/kg), CrO(3) (20mg/kg), and CHL (20mg/kg) 4h before (CHL-CrO(3)) or 4h 
      before and 20h after chromium treatments (20mg/kg; CHL-CrO(3)-CHL). Peripheral 
      blood samples were drawn from the caudal vein at 0, 12 and 48h, and analyzed by 
      the acridine orange (AO) technique. The results obtained in present study shown 
      that CHL injection did not modify the number of MN-PCE. CrO(3) treatment resulted 
      in a significantly increases 12 and 48h after the injection, reaching a four-fold 
      increase 48h after CrO(3) administration. Whereas treatment with 20mg/kg of CHL 
      prior to chromium, decreased the MN frequency induced by chromium in the 12h 
      samples. When the samples were analyzed 48h after CrO(3) injection, no 
      significant differences between CHL-CrO(3) and CHL-CrO(3)-CHL in comparison with 
      CrO(3) treatment, were observed. These results indicate that increase of MN-PCE 
      by CrO(3) is CHL-blocked in both protocols used (CHL-CrO(3) and CHL-CrO(3)-CHL) 
      at 12h after treatment, but it was unable to modify the frequency of MN-PCE 
      measured 48h after CrO(3) injection. The absence of a protective effect by CHL in 
      the MN-PCE induction by CrO(3) at 48h, show that CHL has action only on one of 
      the times of MN induction and suggests the possible action of CrO(3) by two 
      different mechanisms, and not by CHL time-limited in vivo.
FAU - Garcia-Rodriguez, M C
AU  - Garcia-Rodriguez MC
AD  - Laboratorio de Citogenetica, Mutagenesis y Toxicologia Reproductiva, UIBR, 
      Facultad de Estudios Superiores-Zaragoza, UNAM, A.P. 9-020, 15000, D.F., Mexico 
      city, Mexico.
FAU - Lopez-Santiago, V
AU  - Lopez-Santiago V
FAU - Altamirano-Lozano, M
AU  - Altamirano-Lozano M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Chlorophyllides)
RN  - 0 (Chromium Compounds)
RN  - 0 (Mutagens)
RN  - 8LV49809UC (chromium trioxide)
RN  - EEM82VOY7C (chlorophyllin)
SB  - IM
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Cell Count
MH  - Chlorophyllides/*pharmacology
MH  - Chromium Compounds/*toxicity
MH  - DNA Damage/*drug effects
MH  - Female
MH  - Mice
MH  - Micronucleus Tests
MH  - Mutagens/*toxicity
MH  - Reticulocytes/cytology/drug effects
MH  - Time Factors
EDAT- 2001/09/12 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
AID - S138357180100225X [pii]
AID - 10.1016/s1383-5718(01)00225-x [doi]
PST - ppublish
SO  - Mutat Res. 2001 Sep 20;496(1-2):145-51. doi: 10.1016/s1383-5718(01)00225-x.