PMID- 11551659
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20200409
IS  - 0168-1702 (Print)
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 79
IP  - 1-2
DP  - 2001 Nov 5
TI  - Complete genome comparison of porcine reproductive and respiratory syndrome virus 
      parental and attenuated strains.
PG  - 189-200
AB  - Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) 
      genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine 
      strain, were compared and analyzed in order to identify possible sites of 
      attenuation. Of the 41 nucleotide changes, 12 resulted in conservative changes 
      and 18 produced non-conservative changes. The results suggest that key amino 
      acids in ORF1 may contribute to the phenotype of RespPRRS, which includes 
      increased growth rate on MA-104 cells and decreased virulence in swine. The 
      results provide a genetic basis for future manipulation of a PRRSV reverse 
      genetics system.
FAU - Yuan, S
AU  - Yuan S
AD  - Department of Veterinary PathoBiology, 205 Veterinary Science Building, 
      University of Minnesota, 1971 Commonwealth Avenue, 55108, St. Paul, MN, USA.
FAU - Mickelson, D
AU  - Mickelson D
FAU - Murtaugh, M P
AU  - Murtaugh MP
FAU - Faaberg, K S
AU  - Faaberg KS
LA  - eng
PT  - Comparative Study
PT  - Corrected and Republished Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Structural Proteins)
SB  - IM
CRF - Virus Res. 2001 Apr;74(1-2):99-110. PMID: 11226578
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Viral
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - RNA, Viral/analysis
MH  - Sequence Analysis, RNA
MH  - Swine
MH  - Vaccines, Attenuated/genetics
MH  - Viral Structural Proteins/genetics
PMC - PMC7125757
EDAT- 2001/09/12 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/09/04
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
PHST- 2001/09/04 00:00 [pmc-release]
AID - S0168170201002945 [pii]
AID - 10.1016/s0168-1702(01)00295-7 [doi]
PST - ppublish
SO  - Virus Res. 2001 Nov 5;79(1-2):189-200. doi: 10.1016/s0168-1702(01)00295-7.