PMID- 11551935
OWN - NLM
STAT- MEDLINE
DCOM- 20011226
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 46
DP  - 2001 Nov 16
TI  - Differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI) 
      is a vesicular glutamate transporter in endocrine glutamatergic systems.
PG  - 43400-6
AB  - Vesicular glutamate transporter is present in neuronal synaptic vesicles and 
      endocrine synaptic-like microvesicles and is responsible for vesicular storage of 
      L-glutamate. A brain-specific Na(+)-dependent inorganic phosphate transporter 
      (BNPI) functions as a vesicular glutamate transporter in synaptic vesicles, and 
      the expression of this BNPI defines the glutamatergic phenotype in the central 
      nervous system (Bellocchio, E. E., Reimer, R. J., Fremeau, R. T., Jr., and 
      Edwards, R. H. (2000) Science 289, 957-960; Takamori, S., Rhee, J. S., Rosenmund, 
      C., and Jahn, R. (2000) Nature 407, 189-194). However, since not all 
      glutamatergic neurons contain BNPI, an additional transporter(s) responsible for 
      vesicular glutamate uptake has been postulated. Here we report that 
      differentiation-associated Na(+)-dependent inorganic phosphate cotransporter 
      (DNPI), an isoform of BNPI (Aihara, Y., Mashima, H., Onda, H., Hisano, S., 
      Kasuya, H., Hori, T., Yamada, S., Tomura, H., Yamada, Y., Inoue, I., Kojima, I., 
      and Takeda, J. (2000) J. Neurochem. 74, 2622-2625), also transports L-glutamate 
      at the expense of an electrochemical gradient of protons established by the 
      vacuolar proton pump when expressed in COS7 cells. Molecular, biological, and 
      immunohistochemical studies have indicated that besides its presence in neuronal 
      cells DNPI is preferentially expressed in mammalian pinealocytes, alphaTC6 cells, 
      clonal pancreatic alpha cells, and alpha cells of Langerhans islets, these cells 
      being proven to secrete L-glutamate through Ca(2+)-dependent regulated exocytosis 
      followed by its vesicular storage. Pancreatic polypeptide-secreting F cells of 
      Langerhans islets also expressed DNPI. These results constitute evidence that 
      DNPI functions as another vesicular transporter in glutamatergic endocrine cells 
      as well as in neurons.
FAU - Hayashi, M
AU  - Hayashi M
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Okayama 
      University, Okayama 700-8530, Japan.
FAU - Otsuka, M
AU  - Otsuka M
FAU - Morimoto, R
AU  - Morimoto R
FAU - Hirota, S
AU  - Hirota S
FAU - Yatsushiro, S
AU  - Yatsushiro S
FAU - Takeda, J
AU  - Takeda J
FAU - Yamamoto, A
AU  - Yamamoto A
FAU - Moriyama, Y
AU  - Moriyama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010910
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Slc17a6 protein, rat)
RN  - 0 (Vesicular Glutamate Transport Protein 2)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0RH81L854J (Glutamine)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9NEZ333N27 (Sodium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Aspartic Acid/metabolism
MH  - Biological Transport
MH  - Blotting, Northern
MH  - COS Cells
MH  - Calcium/metabolism
MH  - Carrier Proteins/*chemistry/*physiology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - DNA, Complementary/metabolism
MH  - Glutamic Acid/metabolism
MH  - Glutamine/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Islets of Langerhans/metabolism
MH  - Male
MH  - *Membrane Transport Proteins
MH  - Neurons/metabolism
MH  - Pineal Gland/cytology
MH  - Protein Binding
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium/*pharmacology
MH  - Vesicular Glutamate Transport Protein 2
MH  - *Vesicular Transport Proteins
EDAT- 2001/09/12 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
AID - S0021-9258(19)82979-4 [pii]
AID - 10.1074/jbc.M106244200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Nov 16;276(46):43400-6. doi: 10.1074/jbc.M106244200. Epub 2001 
      Sep 10.