PMID- 11551937
OWN - NLM
STAT- MEDLINE
DCOM- 20011226
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 46
DP  - 2001 Nov 16
TI  - The viral CC chemokine-binding protein vCCI inhibits monocyte chemoattractant 
      protein-1 activity by masking its CCR2B-binding site.
PG  - 43270-6
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a chemotactic cytokine mainly 
      acting on monocytes and T cells that elicits its biological effects by 
      interacting with the seven-transmembrane helix receptor CCR2B. The vaccinia virus 
      strain Lister and many other poxviruses express soluble proteins (vCCI) that bind 
      MCP-1 and other CC chemokines and inhibit their function. In order to define the 
      interaction site of MCP-1 with vCCI from vaccinia, surface exposed residues of 
      MCP-1 were identified and mutated to alanine. The MCP-1 variants were expressed, 
      purified, and their interaction with vCCI was characterized. The site on MCP-1 
      for vCCI binding is dominated by arginine 18 with important additional 
      contributions from tyrosine 13 and arginine 24. These residues define a binding 
      site that largely overlaps with the CCR2B receptor interaction site. The viral 
      chemokine-binding protein vCCI thus inhibits the biological function of MCP-1 by 
      directly masking its CCR2B receptor-binding site.
FAU - Beck, C G
AU  - Beck CG
AD  - Department of Arthritis Biology, Novartis Pharma AG, CH-4002 Basel, Switzerland.
FAU - Studer, C
AU  - Studer C
FAU - Zuber, J F
AU  - Zuber JF
FAU - Demange, B J
AU  - Demange BJ
FAU - Manning, U
AU  - Manning U
FAU - Urfer, R
AU  - Urfer R
LA  - eng
PT  - Journal Article
DEP - 20010910
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCI protein, Cowpox virus)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Viral Proteins)
RN  - 0 (Virulence Factors)
RN  - 42HK56048U (Tyrosine)
RN  - 94ZLA3W45F (Arginine)
RN  - OF5P57N2ZX (Alanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alanine/chemistry
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Arginine/chemistry
MH  - Binding Sites
MH  - CHO Cells
MH  - Calcium/metabolism
MH  - Chemokine CCL2/*antagonists & inhibitors/*chemistry
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Kinetics
MH  - Models, Molecular
MH  - Mutation
MH  - Protein Binding
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*chemistry
MH  - Surface Plasmon Resonance
MH  - Two-Hybrid System Techniques
MH  - Tyrosine/chemistry
MH  - Viral Proteins/*chemistry/*metabolism
MH  - Virulence Factors
EDAT- 2001/09/12 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/12 10:00
PHST- 2001/09/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/12 10:00 [entrez]
AID - S0021-9258(19)82963-0 [pii]
AID - 10.1074/jbc.M106305200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Nov 16;276(46):43270-6. doi: 10.1074/jbc.M106305200. Epub 2001 
      Sep 10.